Table 2.
Cells and tissues used in this study. Cells were grown and maintained as directed from the source or as modified (see Methods). Tissues from laboratory or environmentally exposed animals were processed for either tissue sub fractionation, formalin fixation and paraffin embedding followed by immunohistochemistry (IHC), or whole-mount IHC as described in the Methods section. Referenced sources were archived samples from previous studies (see Methods).
| Animal Cell Lines and Tissues | Origin | Source |
|---|---|---|
| ZFL | Zebrafish liver cells | ATCC CRL-2643 |
| PLHC-1 | Top minnow hepatocellular carcinoma | ATCC CRL-2406 |
| Hepa1c1c7 | Mouse liver hepatoma | ATCC CRL-2026 |
| XLK-WG | African clawed frog kidney epithelial | ATCC CRL-2527 |
| HEK293 | Human embryonic kidney | ATCC CRL-3467 |
| Rat liver S9 fraction | Arochlor-1254-induced P450s | MOLTOX 11101.5 |
| Rat liver S9 fraction | Non-induced livers | MOLTOX 11102.2 |
| Recombinant human CYP1A | Over-expressed in HEK293 cell lysates | OriGene LC400170 |
| Mouse livers for IHC | PCB-126-induced CYP1A (and controls) | [51] |
| Rainbow trout liver microsomes & tissue | Β-NF-induced CYP1A (and controls) | [48] |
| Chicken embryo liver | Crude oil induced CYP1A (and controls) | [52] |
| Adult zebrafish for IHC | PCB-126 induced CYP1A (and controls) | This study (see Methods) |
| Larval zebrafish for whole mount IHC | Retene-induced CYP1A (and controls) | This study (see Methods) |
| Gulf killifish for IHC | Tissues from Deep Water Horizon studies | [53] |