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. 2022 Jun 30;14(7):1387. doi: 10.3390/pharmaceutics14071387

Figure 1.

Figure 1

Encapsulation efficiency of histidine-rich peptides: mRNA was complexed to LAH4-L1 (a) or LAH4-L1R (b) at the indicated ratios (µg mRNA: µg peptide) and subjected to treatment with RNase A and proteinase K (indicated with a “+”). mRNA released from the complexes was visualized by gel electrophoresis (representative result, n = 3). The percentage encapsulation efficiency was determined based on the amount of free mRNA after complexation and the mRNA released from the complexes after treatment with proteinase K. mRNA concentration was determined with the Quant-itTM RiboGreen assay kit (n = 2, mean, each dot represents one experiment).