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. 2022 Jun 30;14(7):1387. doi: 10.3390/pharmaceutics14071387

Figure 3.

Figure 3

Histidine-rich peptides allow efficient transfection in DC2.4 cells. DC2.4 cells were transfected with 250 ng of mRNA encoding eGFP per 200,000 cells at the indicated mRNA:HPR ratios (µg:µg). The next day, the cells were harvested, and readout was performed by flow cytometry for percentage (a) and MFI (b) of eGFP (n = 4, mean ± SD). Two-way ANOVA, Tukey’s multiple comparison test: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. DC2.4 cells were transfected at a 1:15 ratio (µg mRNA:µg HRP) with 125 ng of Cy5-labelled mRNA encoding eGFP per 100,000 cells. Readout was performed after 4, 24, and 48 h by flow cytometry for detection of the percentage of Cy5+eGFP+ cells (c) and the MFI of eGFP (d) (n = 3, mean ± SD). Welch’s t-test: * p < 0.05, *** p < 0.001. The positive control (jetMessenger®) and negative controls (without mRNA) are not shown on the graph.