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. 2022 Jun 30;14(7):1387. doi: 10.3390/pharmaceutics14071387

Figure 7.

Figure 7

Transfected CD103+ DCs induce polyfunctionality in OT-I splenocytes ex vivo. Transfected CD103+ DCs were co-cultured with OT-I splenocytes for 3 days. On the second day, monensin and brefeldin A were added overnight to block cytokine secretion. The next day, polyfunctionality was assessed within the CD8+ T-cell population. The 4 functions assessed were proliferation (dilution of CTV) and TNF-α, IFN-γ, and IL-2 production. Negative controls included co-culture with untreated or mock-treated DCs, and positive controls comprised SIINFEKL-pulsed DCs or SIINFEKL directly added to the splenocytes (conditions not shown). CD8+ T cells were assessed for the co-expression of the 4 functions (a) and the percentage of CD8+ T cells producing IL-2 (b). Polyfunctionality was summed up in pie charts, with each part of the whole representing the mean proportion of cells expressing 0–4 functions (c). n = 6; each dot represents one replicate, mean ± SD. One-way ANOVA, Tukey’s multiple comparison test: * p < 0.05, ** p < 0.01.