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. 2022 Jul 16;11(14):2213. doi: 10.3390/cells11142213

Figure 5.

Figure 5

Dichloroacetate enhances glycolytic glucose metabolism in endothelial cells. Primary non-senescent HUVEC were left untreated (control, ctrl) or treated with 2–20 mM dichloroacetate (DCA) for 24 h. (A) Representative western blot of phosphorylated pyruvate dehydrogenase, subunit E1α (p-PDHE1α) at Ser293 and total PDHE1α. (B) Densitometric quantification of p-PDHE1α signals normalized to PDHE1α (n = 4, repeated measure (RM) one-way ANOVA, * p < 0.05 for post-hoc Holm-Šídák test). (C) Lactate levels in medium after 24 h incubation of cells in the presence or absence of DCA (n = 7, RM one-way ANOVA, * p < 0.05 for post-hoc Holm-Šídák test). (D) Glucose consumption as calculated from glucose levels in medium incubated for 24 h in the presence or absence of cells (n = 7, Friedman test, * p < 0.05 for post-hoc Dunn’s test). All data represent mean + SD.