Figure 3.

FAT10, IFI6, IDO1, and GILT are all involved in the antiretroviral activity of γ-IFN. (A) Amphotropic MLV-based vector encoding Renilla luciferase was inoculated to the indicated cells in the absence or presence of γ-IFN. Luciferase activities in control HeLa cells without γ-IFN are always set to 1, and relative values ± SD are indicated. Asterisks indicate significant differences between the indicated groups. This experiment was performed in triplicate. (B) Cell lysates were prepared from indicated cells untreated and treated with γ-IFN and were analyzed by Western blotting. (C) Total RNA samples were prepared from the indicated cells in the absence or presence of γ-IFN. Levels of IFI6, FAT10, GILT, and GAPDH were measured by semi-quantitative RT-PCR.