Table 1.
Nucleotide sequences of primers used in nested PCR.
| Organism (References) | Primers | Sequence (5′ to 3′) | Amplification | Target Gene (Nested Amplification Product Size) |
|---|---|---|---|---|
| Rickettsia [20,21] | RCK/23-5-F | GATAGGTCRGRTGTGGAAGCAC | Primary | 23S-5S target (~350 bp) |
| RCK/23-5-R | TCGGGAYGGGATCGTGTGTTTC | Primary | ||
| RCK/23-5N1F | TGTGGAAG CACAGTAATGTGTG | Nested | ||
| RCK/23-5N1R | TCGTGTGTTTCACTCA TGCT | Nested | ||
| Rickettsia [22] | 17kD1_F | GCTCTTGCAACTTCTATGTT | Primary | 17 kDa protein gene (232 bp) |
| 17kD2_R | CATTGTTCGTCAGGTTGGCG | Primary | ||
| 17kN1_F | CATTACTTGGTTCTCAATTCGGT | Nested | ||
| 17kN2_R | GTTTTATTAGTGGTTACGTAA | Nested | ||
| Rickettsia [23,24] | RpCS.877p | GGGGGCCTGCTCACGGCGG | Primary | gltA (338 bp) |
| RpCS1258n | ATTGCAAAAAGTACAGTGAACA | Primary | ||
| RpCS896p | GGCTAATGAAGCAGTGATAA | Nested | ||
| RpCS1233n | GCGACGGTATACCCATAGC | Nested |