Table 5.

Analytical sensitivity of PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex PCR and the reference PEDV/TGEV/PDCoV PCR by testing serial dilutions of virus isolates.

Dilution	PEDV Isolate IN19338/2013		TGEV Purdue Isolate (VR-763)		PDCoV Isolate (USA/IL/2014)
	5-Plex PCR Ct Value	Reference PCR Ct Value	5-Plex PCR Ct Value	Reference PCR Ct Value	5-Plex PCR Ct Value	Reference PCR Ct Value
10−1	21.09	21.74	21.24	21.32	20.19	21.98
10−1	20.78	21.74	21.35	21.52	20.19	22.27
10−1	20.79	21.75	21.22	21.49	20.29	21.79
10−2	24.41	25.12	25.05	25.22	23.99	25.96
10−2	24.55	25.16	25.02	24.83	23.86	26.05
10−2	24.01	25.27	25.08	25.16	23.98	25.96
10−3	27.85	28.71	28.57	29.32	27.45	29.65
10−3	27.32	29.01	28.98	29.24	27.02	29.45
10−3	27.09	28.88	28.71	29.01	26.98	29.62
10−4	30.50	32.05	31.99	33.05	30.97	32.99
10−4	30.31	32.09	32.34	32.44	30.38	32.73
10−4	29.58	32.01	32.41	32.35	30.15	32.65
10−5	35.86	35.92	35.15	35.68	34.19	35.51
10−5	36.29	37.51	36.06	35.09	33.88	35.89
10−5	35.79	36.48	35.45	36.32	32.96	36.96
10−6	≥40	38.56	≥40	38.54	37.89	≥40
10−6	≥40	38.57	37.29	38.27	36.57	≥40
10−6	36.59	39.56	≥40	38.53	37.21	39.38
10−7	≥40	≥40	≥40	≥40	38.51	≥40
10−7	≥40	39.61	38.63	≥40	≥40	39.11
10−7	≥40	≥40	≥40	≥40	37.57	≥40