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. 2022 Jul 27;10:110. doi: 10.1186/s40168-022-01304-0

Fig. 2.

Fig. 2

DNA-SIP experiments with 13C-labelled DMSP and subsequent separation of heavy and light DNA. a Schematic diagram of SIP experiments with 13C-DMSP and 12C-DMSP (control). b DMSP and DMS levels in seawater samples incubated with 13C- and 12C-DMSP. Autoclaved seawater (AS) was used as abiotic control. Values show the average of three biological replicates. c DNA retrieved as function of refractive index of each fraction recovered after isopycnic ultracentrifugation. Samples in shaded backgrounds were used for downstream analysis. Triangles: seawater samples incubated with 12C-DMSP (control). Circles: seawater samples incubated with 13C-labelled DMSP