Figure 11.

Silencing GPx8 alters ROS levels post-radiation treatment. ROS levels were assessed 20 min, 4 h, and 24 h post-radiation treatment (RT) and compared to its untreated control (0 Gy). Radiation treatment resulted in a significant increase in ROS levels at 20 min (**** p < 0.0001), 4 h (** p = 0.0073), and 24 h (*** p = 0.0004) post-RT. Levels of ROS returned to baseline by 4 h post-RT in the scrambled control, but remained elevated in cells where GPx8 had been silenced. Data are expressed as the mean ± SEM (n = 3). Two-way ANOVA with Tukey’s post-hoc test was adopted for statistical analysis.