Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| RNase inhibitor | Promega | Cat#N2611 |
| 60% Iodixanol solution | Sigma | Cat#D1556 |
| IGEPAL CA-630 | Sigma | Cat#I8896 |
| 35% Bovine Serum Albumin solution | Sigma | Cat#A7979 |
| Sucrose | Millipore | Cat#573113 |
| KCl | Thermo Fisher Scientific | Cat#AM9640G |
| MgCl2 | Thermo Fisher Scientific | Cat#AM9530G |
| PBS | Thermo Fisher Scientific | Cat#10010023 |
| Tris-HCl | Thermo Fisher Scientific | Cat#AM9850G, Cat#AM9855G |
| Tricine | Sigma | Cat#T0377 |
| DTT | Sigma | Cat#646563 |
| Actinomycin | Sigma Aldrich | Cat#A9415 |
| Hoechst 33258 | Invitrogen | Cat#H3569 |
| Capsaicin | Tocris | Cat#0462 |
| Prostaglandin E2 | Tocris | Cat#2296 |
| Bradykinin | Tocris | Cat#3004 |
| Histamine | Sigma | Cat#H7125 |
| KOH | Sigma | Cat#221465 |
| ProLong Gold Antifade Mountant with DAPI | Thermo Fisher | Cat#P36931 |
| RNAscope® Probe-Hs-CD55 | ACD Bio | Cat#426551 |
| RNAscope® Probe-Hs-TAC1 | ACD Bio | Cat#310711-C2 |
| RNAscope® Probe-Hs-SST | ACD Bio | Cat#310591-C3 |
| RNAscope® Probe-Hs-GFRA2 | ACD Bio | Cat#463011 |
| RNAscope® Probe-Hs-KCNS1 | ACD Bio | Cat#487141 |
| RNAscope® Probe-Hs-CALCA | ACD Bio | Cat#605551-C2 |
| RNAscope® Probe-Mm-Sst | ACD Bio | Cat#404631-C3 |
| RNAscope® Probe-Mm-Calca | ACD Bio | Cat#404631-C3 |
| Critical commercial assays | ||
| RNAscope Fluorescent Multiplex Assay | ACD Bio | Cat#320850 |
| Chromium Next GEM Chip G Single Cell Kit | 10X Genomics | Cat#1000127 |
| Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 | 10X Genomics | Cat#1000128 |
| Single Index Kit T Set A | 10X Genomics | Cat#1000213 |
| Chromium Next GEM Chip H Single Cell Kit | 10X Genomics | Cat#1000162 |
| Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 | 10X Genomics | Cat#1000176 |
| 1000212 | 10X Genomics | Cat#1000212 |
| Deposited data | ||
| Raw and analyzed data | This paper | GEO: GSE197289 |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains f | ||
| Mouse: C57BL/6 | The Jackson Laboratory | Cat#000664 |
| Mouse: Sun1-GFP | The Jackson Laboratory | Cat#021039 |
| Mouse: Vglut2-Cre | The Jackson Laboratory | Cat#016963 |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| custom R scripts | This paper | https://github.com/Renthal-Lab/ |
| Fiji | ImageJ | RRID:SCR_002285 |
| Adobe Illustrator CC | Adobe Systems | RRID: SCR_010279 |
| Indrops mapping | Klein et al., 2015 | https://github.com/indrops/indrops |
| Cellranger | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger |
| Cellranger-atac | 10X Genomics | https://support.10xgenomics.com/single-cell-atac/software/pipelines/latest/what-is-cell-ranger-atac |
| R for statistical computing- 4.0.1 | R Core Team, 2018 | https://www.r-proiect.org/ |
| Seurat – 3.2.0 | Satija et al., 2015 | https://satiialab.org/seurat/ |
| Signac – 1.1.0 | Stuart et al., 2020 | https://satiialab.org/signac/ |
| SingleCellSignalR – 0.0.1.8 | Cabello-Aguilar et al., 2020 | https://www.bioconductor.org/packages/release/bioc/html/SingleCellSignalR.html |
| SCENIC – 1.2.2 | Aibar et al., 2017 | https://aertslab.org/#scenic |
| edgeR – 3.30.3 | Robinson et al., 2009 | http://bioinf.wehi.edu.au/edgeR/ |
| ggplot2 – 3.3.2 | Wickham, 2016 | https://ggplot2.tidyverse.org/ |
| gplots – 3.0.4 | Warnes et al., 2009 | https://github.com/talgalili/gplots |
| topGO – 2.40.0 | Alexa, 2018 | https://bioconductor.org/packages/release/bioc/html/topGO.html |
| Other | ||
| Resource website for this publication | This paper | https://tg.painseq.com |