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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Nature. 2022 Jul 20;607(7920):769–775. doi: 10.1038/s41586-022-04896-7

Extended Data 13: ZBP1 activation results in RIPK1 dependent, RIPK3 independent gene transcription which is enhanced by knockout of Casp8.

Extended Data 13:

A-B Differential transcript analysis of Mlkl−/−::Casp8+/+ and Mlkl−/−::Casp8−/− MEFs (n=3) following ADAR depletion, by (A) Heatmap analysis and (B) individual quantification of the top 5 differentially expressed genes. Statistical significance determined by individual unpaired t tests (two-tailed). Where not indicated: **** p<0.0001. C. QPCR analysis of top 5 differentially expressed genes identified (B) in MEFs expressing ZBP1–2xFV after B/B activation after depletion of RIPK1. n=6 biologic replicates for each treatment group, each representing the average of three technical replicates. Data is compiled from two independent experiments. Statistical significance determined by individual unpaired t tests (two-tailed). Where indicated: **** p<0.0001. Whisker bars represent the mean +/− SD.