Figure 3.

SARS-CoV spike responses engage multiple epitopes, whereas N332-GT2 responses are highly epitope focused

(A) Summary of nsEMPEM analysis of human serum samples from four patients naturally infected with SARS-CoV-2.

(B) Summary of nsEMPEM analysis of NHP serum samples from four subjects after immunization by NVX-CoV2373.

(C) Schematic of experimental design to evaluate serum in WT and BG18^gH-recipient mice 14 and 42 days post N332-GT2 immunization.

(D) N332-GT2 binding serum IgG of WT B6 (gray) and BG18^gH recipients (WT+BG18^gH, red) at 14 and 42 dpi. AUC = area under the ELISA binding curve. p values were calculated by unpaired Student’s t test (^∗∗∗∗p < 0.0001; ns, not significant). Data from one experiment with 6–7 mice per condition and presented as mean ± SD.

(E) Summary of nsEMPEM analysis of pooled mouse serum samples from (D). Composite models represent polyclonal antibody targeting against the N332-GT2 trimer. (Purple) Fabs targeting the base of the trimer; (green) Fabs targeting the V1/V3 region.

See also Figure S5.