Figure 6.

High-affinity antigen-specific antibody can restrict corresponding B cell populations from participating during co-evolution

(A) Schematic of experiment to test the antibody-mediated restriction of immunogen-specific responses during co-evolution.

(B) Representative FACS plots at 10 dpi of gating strategy to identify the effects of administration of no antibody, 10 μg of CLK09, or BG18_iGL0 mAb in BG18^gH-GFP/CLK09 double recipients immunized with either N332-GT2 or eOD-GT8 or both (C–E).

(C and D) (C) GC cells as the percentage of total B cells and (D) CD45.2⁺ population as the percentage of total GC B cells at 10 dpi.

(E) Distribution of the CD45.2+ GC B cell populations between BG18^gH-GFP (blue) and CLK09 (red) cells at 10 dpi.

p values were calculated by unpaired Student’s t test (^∗∗p < 0.01; ^∗∗∗∗p < 0.0001). Figures represent data from one of at least two experiments with 2–5 mice per condition, with data presented as mean ± SD.