Figure 7.

Excess antigen can overcome antibody-mediated restriction

(A) Schematic of experiment to evaluate the antigen-mediated depletion of circulating antibodies.

(B and C) (B) ELISA quantification of N332-GT2-specific human IgG and (C) N332-GT2-specifc murine IgG from mice receiving 10 μg BG18_iGL0 and subsequently immunized (blue) or left unimmunized (red) or mice that were immunized without receiving any mAb (black). p values were calculated by unpaired Student’s t tests comparing red versus blue (B) or blue versus black (C) groups at individual time points (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗∗p < 0.0001).

(D) Schematic of experiment to test the effects of multiple doses of antigen on naive CLK09 B cell responses in CLK09 recipients receiving a single dose or triple doses of eOD-GT8 immunogen after receiving either 10 μg CLK09 mAb (red) or no mAb (gray) (E and F).

(E and F) (E) GC cells as the percentage of total B cells and (F) CD45.2⁺ CLK09 cells as the percentage of total GC B cells at 10 dpi of CLK09 recipients.

Figures represent data from one of at least two experiments with 3–5 mice per condition, with data presented as mean ± SD.

See also Figure S7.