REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Hamster monoclonal anti-mouse CD95 PE-Cy7 (clone: Jo2)	BD Biosciences	CAT#:557653
Rat monoclonal anti-mouse CD38 A700 (clone: 90)	Invitrogen	CAT#:56-0381-82
Mouse monoclonal anti-mouse CD45.2 PE (clone: 104)	Biolegend	CAT#:109808
Mouse monoclonal anti-mouse CD45.1 PerCP Cy5.5 (clone: A20)	Biolegend	CAT#:110728
Mouse monoclonal anti-mouse B220 A488 or BV421 (clone: RA3-6B2)	BD Biosciences	CAT#:557669, 562922
Rat monoclonal anti-mouse CD138 BV650 (clone:281-2)	BD Biosciences	CAT#:564068
Rat monoclonal anti-mouse IgD APC-Cy7	Biolegend	CAT#:405716
Rat monoclonal anti-mouse-CD16/32 purified (clone 2.4G2)	BD Biosciences	CAT#:553142
Rat monoclonal anti-mouse CD4 APC-eF780 (clone: RM4-5)	Invitrogen	CAT#:47-0042-80
Rat monoclonal anti-mouse CD8 APC-eF780 (clone: 53-6.7)	Invitrogen	CAT#:47-0081-80
Rat monoclonal anti-mouse F4/80 APC-eF780 (clone: BM8)	Invitrogen	CAT#:47-4801-80
Rat monoclonal anti-mouse Ly-6G APC-eF780 (clone: RB6-8C5)	Invitrogen	CAT#:47-5931-80
Monoclonal BG18_iGL0	Produced in house	N/A
Monoclonal BG18_Pre5	Produced in house	N/A
Monoclonal BG18_Pre14	Produced in house	N/A
Monoclonal muBG18_d42.10	Steichen et al., 2019	N/A
Monoclonal PGT128	Walker et al., 2011	AB_2491047
Monoclonal VRC01	Wu et al., 2010	AB_2491019
Monoclonal PGT145	Walker et al., 2011	AB_2491054
Monoclonal RM19R	Cottrell et al., 2020	N/A
Rabbit polyclonal anti-His	GeneScript	CAT#A00174
Human monoclonal CR3022 SARS-CoV-2 Spike Glycoprotein S1	Abcam	CAT#:ab273073
Alkaline Phosphatase AffiniPure Goat Anti-mouse IgG	Jackson Immuno Research	CAT#:115-055-071
Alkaline Phosphatase AffiniPure Goat Anti-human IgG	Jackson Immuno Research	CAT#:109-055-098
Biological samples
Human plasma samples A (1988), B (1989) and C(1992) from Bangaru et al., 2022; Sample D from study PMID: 32540903	Bangaru et al., 2022; ongoing study	N/A
Immunized rhesus macaque serum	Novavax	N/A
Chemicals, peptides, and recombinant proteins
Streptavidin-A488	Biolegend	CAT#:405235
Streptavidin-PE	Biolegend	CAT#:405204
Streptavidin-A647	Biolegend	CAT#:405237
Streptavidin-BV421	Biolegend	CAT#:405226
LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation	Thermo Fisher Scientific	CAT#: L34962
Sigma Adjuvant	Sigma	CAT#:S6322
Alhydrogel adjvant 2%	Invivogen	CAT#:Vac-alu-250
Papain	Sigma Aldrich	CAT #: P3125-100mg
N332-GT2 ferritin nanoparticle	Steichen et al., 2019	N/A
N332-GT2 trimer	Steichen et al., 2019	N/A
eOD-GT8 60mer immunogen	Jardine et al., 2015	N/A
SARS-CoV-1 RBD monomers	Produced in house	N/A
SARS-CoV-2 Spike Proteins	Produced in house	N/A
SIGMAFAST p-Nitrophenyl phosphate Tablets	Sigma	CAT#: N2770-50SET
Gentle Ag/Ab binding buffer	Pierce	CAT#:21012
Gentle Ag/Ab elution buffer	Pierce	CAT#:21013
ACK lysing buffer	Lonza	CAT#:10-548E
Monomeric and trimeric SARS-CoV-2 and SARS-CoV receptor binding domains	This paper	N/A
TALON Metal Affinity Resin	Takara	CAT#635652
ExpiFectamine 293 Transfection Kit	Thermo Fisher	Cat#A14525
Critical commercial assays
Pan B Cell Isolation Kit II, mouse	Miltenyi Biotec	CAT#:130-104-443
UltraComp eBeads™ Compensation Beads	Thermo Fisher Scientific	CAT#:01-2222-42
Protein G chromatography cartridges, 5ml	Pierce	CAT#:89927
PD-10 desalting columns	GE Life Sciences	CAT#:17085101
Deposited data
SARS-CoV-2 spike protein complexed with polyclonal Fab Human donor A Human donor B Human donor C Human donor D	This paper	EMDB: EMD-27200 EMDB: EMD-27199 EMDB: EMD-27198 EMDB: EMD-27197
S protein mix + NVX-vaccinated NHPs 36057 36964 37452 37611	This paper	EMDB: EMD-27039 EMDB: EMD-27040 EMDB: EMD-27041 EMDB: EMD-27042
N332-GT2 + Mouse Fab BG18, d14 WT, d42 BG18, d42	This paper	EMDB: EMD-27036 EMDB: EMD-27037 EMDB: EMD-27038
BCR sequences for CR3022Gl	This paper	GenBank: OP079065– OP079171; GenBank: OP079441–OP079547
BCR sequences for CR3022Ma	This paper	GenBank: OP079172–OP079380; GenBank: OP079548–OP079756
BCR sequences for CC12.1	This paper	GenBank: OP079381–OP079440; GenBank: OP079757–OP079826
Experimental models: Cell lines
HEK-293T-F	Thermo Fisher Scientific	Cat#: R79007
Human: Expi293F	Thermo Fisher	Cat#A14527; RRID: CVCL_D615
Experimental models: Organisms/strains
Mouse: B6.SJL-Ptprcapepcb/BoyJ	The Jackson Laboratory	JAX:002014
Mouse: C57BL/6J	The Jackson Laboratory	JAX:000664
Mouse : BG18gH	Lin et al., 2018	N/A
Mouse : BG18gH-GFP	This paper	N/A
Mouse : MD4	The Jackson Laboratory; Mason et al., 1992	JAX:002595
Mouse : CR3022Gl	This paper	N/A
Mouse CR3022Ma	This paper	N/A
Mouse CC12.1	This paper	N/A
Mouse : CLK09	Wang et al., 2021a	N/A
Mouse : CLK19	Wang et al., 2021a	N/A
Oligonucleotides
sgRNA	Lin et al., 2018; Wang et al., 2021a	N/A
Recombinant DNA
Plasmids for CR3022Gl, Ma and CC12.1	Produced in house	N/A
Software and algorithms
FlowJo 10.8.1	Treestar	https://www.flowjo.com/
Prism 8.4.3	GraphPad	https://www.graphpad.com/
Leginon	Suloway et al., 2005	https://emg.nysbc.org/redmine/projects/leginon/wiki/Leginon_Homepage
Appion	Lander et al., 2009	N/A
DogPicker	Voss et al., 2009	N/A
RELION	Scheres et al., 2012	https://relion.readthedocs.io/en/release-3.1/
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
Other
BD LSRfortessa	BD Biosciences	N/A
BD FACS ARIA II instrument	BD Biosciences	N/A
0.5 ml -10 kDa concentrator	Amicon Ultra	CAT #: UFC803096
15 ml-10 kDa concentrator	Amicon Ultra	CAT #: UFC901024
Protein G Sepharose 4 Fast Flow	Cytiva	CAT #: 17127903
AKTA pure system	Cytiva	N/A
FEI Tecnai Spirit T12 transmission electron microscope		N/A
TemCam F416 CMOS 4k x 4k camera	TVIPS	N/A
BioTek Synergy Neo2	Biotek	N/A
Biotek EL406 washer/dispenser	Biotek	N/A
Superdex 200 Increase 10/300 GL	GE Healthcare	Cytiva 28-9909-44