Fig. 2.
Examples of functional microscopy data. Functional microscopy data obtained via different modalities, at different scales, and in different animal models expressing different indicators can take on a diversity of signal characteristics. Shown are six examples spanning a range of datasets. (a) Laser scanning two-photon calcium imaging (GCaMP5) in mouse hippocampus.49 (b) Glutamate (iGluSnFR3.v857) spine imaging using laser-scanning two-photon microscopy in layer two-third mouse cortex.50 (c) One-photon widefield calcium (GCaMP6f) imaging of rat dorsal cortex using a head-mounted macroscope.48 (d) One-photon calcium imaging using a head-mounted microendoscope in mouse striatum.51 (e) One-photon voltage imaging (Voltron525-ST) in layer 1 of mouse cortex.52 (f) Whole-brain light-sheet calcium imaging (GCaMP6f) in larval zebrafish.53 Only one of 29 imaging planes each recorded at 2 Hz is depicted here. Note that the spatial and temporal statistics range dramatically across domains, requiring flexible data processing approaches.