Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1999 Mar;181(6):1728–1732. doi: 10.1128/jb.181.6.1728-1732.1999

A Cold Shock-Induced Cyanobacterial RNA Helicase

Danuta Chamot 1, Wendy C Magee 1,, Esther Yu 1, George W Owttrim 1,*
PMCID: PMC93569  PMID: 10074063

Abstract

The ability to modify RNA secondary structure is crucial for numerous cellular processes. We have characterized two RNA helicase genes, crhB and crhC, which are differentially expressed in the cyanobacterium Anabaena sp. strain PCC 7120. crhC transcription is limited specifically to cold shock conditions while crhB is expressed under a variety of conditions, including enhanced expression in the cold. This implies that both RNA helicases are involved in the cold acclimation process in cyanobacteria; however, they presumably perform different roles in this adaptation. Although both CrhB and CrhC belong to the DEAD box subfamily of RNA helicases, CrhC encodes a novel RNA helicase, as the highly conserved SAT motif is modified to FAT. This alteration may affect CrhC function and its association with specific RNA targets and/or accessory proteins, interactions required for cold acclimation. Primer extension and analysis of the 5′ untranslated region of crhC revealed the transcriptional start site, as well as a number of putative cold shock-responsive elements. The potential role(s) performed by RNA helicases in the acclimation of cyanobacteria to cold shock is discussed.


RNA helicases are single-stranded RNA-dependent ATPases that convert double-stranded RNA into single-stranded RNA through unwinding activity. These enzymes have been implicated in a diverse range of cellular processes including ribosome biogenesis, translation initiation, cell growth and differentiation, oogenesis, and oncogenesis (5, 28). RNA helicases comprise three related families of proteins based on the spatial and sequence conservation of eight amino acid motifs, including DEAD, DEAH, and DEXH, from which the family names are derived (6). Theoretically, RNA helicases may be involved in any process in which modulation of RNA secondary structure is required. In fact, putative RNA helicase-encoding gene sequences are ubiquitous, having been identified in organisms ranging from bacteria to humans, as well as in positive-strand RNA viruses (5, 28).

Although sequences encoding putative RNA helicases have been identified in a number of prokaryotic species, including those of the genus Bacillus (GenBank accession no. P42305), the domain Archaea (30), and the genus Synechocystis (12), they have been studied most extensively for Escherichia coli, whose genome encodes several RNA helicases belonging to all three families. Functions that have been identified for E. coli DEAD box RNA helicases include 23S rRNA function (dbpA [17]), ribosome biogenesis (srmB [18]), RNA turnover (rhlB [23]), and cold shock adaptation (csdA [9]). csdA expression increases upon a temperature shift from 37 to 15°C, with the 70-kDa CsdA protein being ribosome associated at low temperatures and possessing helix-destabilizing activity (9). A function has not been ascribed to a fifth E. coli DEAD box RNA helicase, rhlE; however, a null mutant grows normally under a variety of conditions, implying that rhlE is not an essential gene (19).

RNA helicases have not been studied to date in the diverse, heterogeneous group of gram-negative photosynthetic prokaryotes, the cyanobacteria (29). Cyanobacteria are an excellent model system in which to study RNA helicase function as they are the ancestors of modern chloroplasts (7) and, in addition, perform a number of complex physiological phenomena involving cellular differentiation, such as aerobic nitrogen fixation and akinete formation (29). Evidence from other systems implicates RNA helicases in similar differentiation processes (5, 28).

Here we report the first molecular characterization of RNA helicase genes from a cyanobacterium, the filamentous, nitrogen-fixing cyanobacterium, Anabaena sp. strain PCC 7120 (referred to hereafter as Anabaena). The results indicate that Anabaena contains a minimum of two divergent RNA helicase genes; crhB, which exhibits expression under a broad range of conditions, and the novel RNA helicase gene, crhC, which is specifically expressed only under cold shock conditions.

MATERIALS AND METHODS

Organisms and culture conditions.

Axenic cultures of Anabaena sp. strain PCC 7120, obtained from the University of Toronto Culture Collection (UTCC 387), were grown photoautotrophically at 30°C in BG-11 medium with a 16-h-light–8-h-dark cycle. Illumination was provided by fluorescent lamps at 150 microeinsteins m−2 s−1. Aeration was provided by continuous bubbling with air and shaking on a rotary shaker at 150 rpm.

DNA manipulation.

Standard methods, as described elsewhere (26), were utilized for DNA manipulations including Southern blotting and in situ colony hybridization with the cloning vector pBluescript KS(+) (Stratagene) and the E. coli host, DH5α. For Southern blot analysis, genomic DNA, isolated from Anabaena (10), was transferred to a Hybond N membrane (Amersham). Hybridization was performed overnight at 60°C with either the crhB or the crhC PCR products labelled with [32P]dCTP (Amersham) with a random-primer labelling kit (New England Biolabs). DNA fragments were sequenced on both strands with Sequenase version 2.0 (Amersham). DNA sequence analysis was performed with the University of Wisconsin Genetics Computer Group Sequence Analysis Software (GCG) programs, version 8.1.

PCR amplification.

To isolate RNA helicase-encoding sequences, three degenerate PCR primers, based on conserved amino acid motifs found in five E. coli DEAD box RNA helicases (11), were synthesized (R as purine, Y as pyrimidine, and N as any of the four nucleotides): 5′-RTNYTNGAYGARGCNGA-3′ from the conserved motif VLDEAD, 5′-CCNACN(C/A)GNGARYTNGC-3′ from the conserved motif PTRELA, and 5′-GCNGCNACRTCNGTNGC-3′ from the conserved motif ATDVAA. PCRs were performed in a reaction volume of 50 μl containing approximately 0.5 U of Taq DNA polymerase and each of the primers at a 1 μM concentration. The PCR program consisted of a 3-min denaturation-incubation at 94°C, followed by 20 cycles of 1 min of denaturation at 92°C, 1 min of primer annealing at 70°C, and 1.5 min of primer extension at 72°C and then by 20 cycles of 1 min of denaturation at 92°C, 1 min of primer annealing at 60°C, and 1.5 min of primer extension at 72°C, and terminated with a final incubation of 5 min of primer extension at 72°C. The annealing temperature was initially 70°C and was dropped by 0.5°C per cycle during the first 20 cycles. The correct-sized PCR products were purified from agarose gels and ligated into pCRII (Original TA cloning kit; Invitrogen). Cloned PCR products were subjected to Southern blot hybridization with the tobacco DEAD box RNA helicase gene, NeIF-4A2 (20), as probe.

Cloning Anabaena crh genes.

Libraries containing XbaI-digested Anabaena genomic DNA ranging from 3 to 5 and 5 to 10 kb were constructed by ligation into XbaI-digested and dephosphorylated pBluescript KS(+) (Stratagene). The libraries were first screened by Southern blot hybridization with either the cloned crhB or crhC PCR fragments, followed by in situ colony hybridization (26).

Northern analysis.

Total Anabaena RNA was isolated from liquid cultures (27) grown under the stated conditions. Denatured RNA (15 μg) was size fractionated on 1.2% formaldehyde-agarose gels and transferred to a Hybond N+ membrane (Amersham) (26). Hybridization was performed overnight at 65°C in 50% formamide–5× SSPE (1× SSPE is 0.18 M NaCl, 10 mM NaH2PO4, and 1 mM EDTA [pH 7.7])–0.2% sodium dodecyl sulfate (SDS)–5× Denhardt’s solution with antisense probes generated from either the crhB or the crhC PCR-cloned products labelled with [32P]UTP (Amersham) with a Riboprobe kit (Promega). A 500-bp EcoRI-HindIII fragment encoding the Anabaena RNase P gene was radiolabelled as described for DNA manipulations and used to probe Northern blots overnight at 65°C in aqueous buffer according to the manufacturer’s protocol (Amersham). Blots were washed for 15 min at 65°C once in 1× SSPE–0.1% SDS and twice in 0.1× SSPE–0.1% SDS. Blots were stripped and reprobed according to the manufacturer’s protocol.

Primer extension analysis of the crhC transcript.

Total RNA was isolated from Anabaena cells grown under cold shock-inducing conditions for 3 h, as described above. Primer extension reactions were performed (26), with 50 μg of RNA and 6 × 105 cpm of [γ-32P]ATP end-labelled primer. The primer was a 19-base oligonucleotide, 5′-CGTCCTGATAAGACAGCAG-3′, corresponding to the noncoding strand of the crhC gene beginning 98 nucleotides downstream of the initiator ATG. Primer extension products were separated on 6% sequencing gels in parallel with sequencing reactions of the crhC gene performed with the same primer.

Nucleotide sequence accession numbers.

The nucleotide sequences reported in this study have been submitted to the EMBL data bank under the accession no. AF040044 (crhB) and AF040045 (crhC).

RESULTS

PCR cloning of RNA helicase gene fragments from Anabaena.

Sequencing of cloned PCR products which hybridized with the tobacco DEAD box RNA helicase gene, NeIF-4A2 (20), revealed three clones with significant sequence similarity to DEAD box RNA helicases. Two of the clones contained identical 758-bp inserts, named crhC (for cyanobacterial RNA helicase cold), while the third contained a 348-bp insert, named crhB. crhC and crhB encode distinct RNA helicase-related amino acid sequences. CrhB exhibits 32% identity and 51% similarity at the deduced amino acid level to the corresponding region of CrhC. crhC is a novel RNA helicase gene, as it encodes a FAT box, a unique alteration of the strictly conserved SAT motif, found in all but one other DEAD box RNA helicase.

Gene copy number determination.

Southern blot analysis indicated that both crhB and crhC genes are present as single distinct gene copies in the Anabaena chromosome (Fig. 1).

FIG. 1.

FIG. 1

Southern blot analysis of Anabaena sp. strain PCC 7120 crh genes. Southern blots of digested Anabaena genomic DNA (3 μg) were hybridized at 60°C with either the crhC (A) or the crhB (B) PCR fragment. The autoradiograms are shown. Restriction enzymes utilized were ClaI (lanes 1), EcoRI (lanes 2), HindIII (lanes 3), and XbaI (lanes 4). The positions of lambda HindIII molecular mass markers (kilobases) are shown to the left.

Cloning and sequence analysis of a crhC genomic clone.

Two full-length crhC clones, containing identical 4.3-kb inserts, were isolated from size-fractionated XbaI genomic libraries with the cloned crhC PCR fragment as a probe. A physical map of one crhC clone, pWM75, indicated that the open reading frame was located on an internal HincII-EcoRV fragment (data not shown). Sequence analysis indicated that this 1,964-bp fragment contained a 1,275-bp open reading frame, encoding a 425-amino-acid polypeptide having a predicted molecular mass of 47,481 Da and a pI of 10.79 (data not shown). CrhC contains seven of the conserved amino acid motifs (Fig. 2, thick bars) diagnostic of all DEAD box RNA helicase proteins in terms of both sequence and spatial orientation. The eighth motif, the SAT box, is present as a FAT motif in CrhC (Fig. 2, arrowhead).

FIG. 2.

FIG. 2

Multiple amino acid sequence alignment of CrhC with known and putative RNA helicases. Sequence sources (EMBL GenBank accession numbers in parentheses) are CrhC from Anabaena (AF040045); RhlE from E. coli (P25888); 6803, slr0083 from Synechocystis sp. strain PCC 6803 (D64004); DbpA from E. coli (P21693); CsdA from E. coli (P23304); RhlB from E. coli (P24229); SrmB from E. coli (P21507); Tif1 from Saccharomyces cerevisiae (X12813); and N4A2, NeIF-4A2 from tobacco (X61205). The eight diagnostic DEAD box amino acid motifs are shown above the sequence by thick bars. The consensus line designates identical amino acids or conservative changes in seven of nine proteins as follows: r, branched aliphatic amino acids, I or L or V; a, acidic amino acids, D or E; b, basic amino acids, R or K; p, aliphatic unbranched amino acids, G or A; h, hydroxyl side chain amino acids, T or S; and m, aromatic amino acids, Y or F. An arrowhead indicates the FAT motif.

The deduced Anabaena CrhC gene product exhibits similarity to a number of established and putative RNA helicases (Fig. 2). CrhC is most closely related to the E. coli RNA helicase, RhlE, having 74% similarity (60% identity). The partial CrhB sequence exhibits the highest similarity, 70% (64% identity), with the corresponding region, amino acids 153 to 268, of a putative RNA helicase gene in Synechocystis sp. strain PCC 6803 (12) (data not shown). CrhB and CrhC are as equally related to other DEAD box RNA helicase sequences with which they have 55 to 60% similarity (36 to 39% identity) as they are to each other.

Numerous attempts to isolate a full-length crhB genomic clone were not successful, apparently as a result of toxicity of the cloned gene in E. coli.

crh gene expression.

Northern blot analysis indicates that the crhC transcript is specifically expressed in cells which have been temperature shifted from 30 to 20°C, i.e., under cold shock conditions (Fig. 3A, lane 2). The crhB transcript was differentially expressed under any condition in which the cells were grown in the light, including stimulation by cold shock (Fig. 3B, lane 2). RNase P hybridization indicates that the presence or absence of hybridizing transcripts for crhC or crhB is not due to RNA loading (Fig. 3C).

FIG. 3.

FIG. 3

Northern analysis of crhC and crhB expression. A Northern blot of total RNA extracted from Anabaena grown under the stated conditions was hybridized with crhC (A). The blot was consecutively stripped and probed with crhB (B) followed by the Anabaena RNase P gene (C). The resulting autoradiograms are shown. Lane 1, 22 h in the absence of fixed nitrogen; lane 2, 3 h at 20°C; lane 3, 30 min at 44°C; lane 4, 3 min of UV illumination, followed by 3 h in the dark; lane 5, 3 h in the presence of 0.02% methanesulfonic acid methyl ester; lane 6, 3 h in the presence of 80 μg of nalidixic acid per ml; lane 7, 3 h in the presence of 5 μg of mitomycin C per ml; lane 8, 3 h in the presence of 0.5 M NaCl; lane 9, 3 h in the dark; lane 10, cells grown continuously at 30°C in the light. Cells for the experiments whose results are shown in lanes 1, 2, and 10 were grown continuously in the light, while cells were grown in the light and transferred to the dark during the course of the experiments whose results are shown in lanes 3 to 9.

Identification of the crhC transcription start site.

To identify the promoter region responsible for cold shock induction of crhC transcription, total RNA isolated from cold shock-induced Anabaena cells was subjected to primer extension analysis. Total RNA from noninduced cells was also subjected to primer extension analysis as a control. A primer extension product was detected only in RNA isolated from cold shock-induced cells, as an intense GG doublet (Fig. 4, lane 2). The proximal G residue, located 115 nucleotides upstream of the crhC translational start codon, was designated as the transcriptional start site (Fig. 5, asterisk). A minor signal corresponding to a C residue 120 nucleotides from the crhC translational start codon was also observed. Although the importance of this second potential transcription initiation site is not known, a variety of cyanobacterial genes have been shown to have multiple start sites (3). A primer extension product was not observed in RNA isolated from non-cold-shock-induced cells (Fig. 4, lane 1), in agreement with the Northern blot analysis (Fig. 3A).

FIG. 4.

FIG. 4

Primer extension analysis of the transcription start site. An autoradiogram shows primer extension experiments performed with 50 μg of RNA extracted from Anabaena grown continuously at 30°C (lane 1) or for 3 h at 20°C (lane 2). Lanes A, C, G, and T are sequencing reactions performed on crhC with the primer extension oligonucleotide. An arrow indicates the hybridizing extension product corresponding to the G residue taken to be the start of transcription.

FIG. 5.

FIG. 5

Sequence analysis of putative crhC transcriptional-translational elements. Shown is the DNA sequence surrounding the ATG initiator methionine codon. Numbering reflects the transcription start site determined by primer extension and taken to be +1 (indicated by an asterisk). Putative regulatory elements are indicated as follows: boldface, −10 region; underlining, AT-rich upstream element; thickly outlined box, cold shock box; double underlining, Shine-Dalgarno sequence; and thinly outlined box, downstream box.

Sequence analysis of putative crhC regulatory elements.

Analysis of the crhC promoter for transcriptional elements indicates that a ς70-dependent −10-like sequence, TAAGAT, is present beginning 9 nucleotides upstream of the transcription start site (Fig. 5, boldface). A −35-like region within the space constraints of typical E. coli promoters was not observed, as expected for a gene which is not expressed under normal growth conditions. An AT-rich sequence resembling an upstream enhancer element (16) is located at positions −53 to −65 (Fig. 5, underlining). A putative E. coli-like cold shock box involved in transcriptional attenuation is located at positions +87 to +97 (Fig. 5, thickly outlined box). The Anabaena cold shock box, 5′-TGACAGGCCGA-3′, matches the E. coli cold shock box (5′-TGACGTACAGA-3′) (8) at 7 of 11 positions.

Analysis of the sequences flanking the initiator methionine residue (position +116 from the transcription initiation site) for translational activators indicates that the 5′ untranslated region (UTR) possesses a putative E. coli-like Shine-Dalgarno sequence 3 nucleotides upstream of the translation initiation codon with six of eight residues base pairing (Fig. 5, double underlining) with the Anabaena 16S rRNA (14). The 16S rRNA-crhC mRNA base-pairing potential continues downstream from this site, through the translation initiation codon, up to a 15-nucleotide sequence beginning at position +137, in which 9 of 15 nucleotides are base paired (Fig. 5, thinly outlined box). This region strongly resembles a downstream box (16) involved in the translational activation of cold shock-induced E. coli genes. The 5′ region of crhC from −115 to +71 is also predicted to fold into a complex, stable secondary structure, with a ΔG° of −35.4 kcal/mol (data not shown). In this structure, the AUG is located in a loop while the proposed Shine-Dalgarno sequence and the downstream box are partially base paired.

The 3′ UTR contains a 22-nucleotide stem-loop sequence corresponding to a weak rho-independent transcriptional terminator consisting of an 8-bp GC-rich stem and a 6-base loop structure followed by a string of four T residues. This potential transcriptional terminator is located 26 nucleotides downstream of the translational stop codon (data not shown). Similar sequences have been shown to be involved in transcription termination and/or mRNA stability in prokaryotic organisms (24).

DISCUSSION

We report the first molecular characterization of cyanobacterial RNA helicase genes. The results indicate that the Anabaena genome possesses two distinct genes, as confirmed by Southern and Northern analyses. At this point, we cannot discount the possibility that additional, more divergent cyanobacterial RNA helicases exist in Anabaena, since crhC and crhB do not cross-hybridize. Divergent, multimember DEAD box RNA helicase gene families exist in other organisms, including E. coli (11) and tobacco (1, 20, 21).

Deduced amino acid sequence analysis did not provide clues to the function performed by the crhC gene product, as the most closely related gene, the E. coli rhlE gene, has not been characterized. Amino acid analysis did reveal, however, that CrhC is a novel DEAD box RNA helicase, as it possesses a FAT box instead of a diagnostic SAT box. The Ser-to-Phe modification can be accounted for by a C-to-T transition, from a TCC Ser codon to a TTC Phe codon. DEXH box RNA helicases also contain a modified SAT box, a TAT motif (5). In this case, however, the replacement of Ser with Thr is conservative, as both amino acids are polar and uncharged. A putative tobacco DEAD box RNA helicase, NeIF-4A12, also contains a modified SAT motif, a YAT box (1). In this case, the Ser-to-Tyr modification can also result from a transition at the second base of the codon. Interestingly, in both NeIF-4A12 and CrhC, the modified amino acid is aromatic and thus hydrophobic, a significant departure from the polar, uncharged Ser or Thr residues normally present at this position.

The FAT alteration may have significant effects on the mechanism by which CrhC functions. In vitro and in vivo mutational analyses of mammalian and yeast eIF-4A have implicated the SAT box in coupling the ATPase and RNA helicase activities (22, 28). Crystal structure analysis of a viral DEXH RNA helicase supports these results (2) and led to the proposal that the TAT motif acts as a flexible hinge linking the active sites for ATPase and helicase activities. This would involve hydrogen bond switching between the His residue in the DEXH motif, which is involved in the ATPase activity, and either of the two Thr residues present in the TAT motif, involved in RNA helicase activity. This scenario would not be possible in CrhC, as Phe is not capable of hydrogen bond formation. This implies a more rigid CrhC protein which may limit the RNA substrates with which it can interact.

It is interesting that crhC expression is specifically induced upon a downshift in temperature of 10°C but is not induced by a broad range of other stress conditions. This indicates that crhC is not a general stress-induced gene. The cold shock-specific expression of crhC is similar to that of the E. coli RNA helicase gene csdA (9); however, CrhC is not a CsdA homologue, as CrhC most closely resembles the E. coli RNA helicase RhlE. This implies that cold acclimation in prokaryotes involves RNA helicase activity; however, the helicase need not be conserved between genera.

The cold shock-induced gene expression of crhC may be regulated by a number of elements in both the promoter and the translation initiation codon regions. On the transcriptional level, crhC contains both an AT-rich upstream element which functions as a transcriptional activator for cspA in E. coli (16) and a cold shock-like box which is involved in transcriptional attenuation of cspA in E. coli (8). On the translational level, crhC may be regulated negatively by its relatively long, highly structured 5′ UTR (115 nucleotides), similar to that observed in the extended autoregulatory 5′ UTRs found in the E. coli cold shock-induced genes, cspA, cspB, and csdA (4, 8). crhC also encodes a downstream element complementary to the 3′ end of the 16S rRNA. Similar downstream elements are essential for translation of cold shock-induced mRNAs in E. coli (16).

The cold shock induction of RNA helicase gene expression represents a novel gene family which is induced upon cold acclimation in cyanobacteria. Other cyanobacterial gene families that have been shown to be cold shock induced include those encoding RNA-binding proteins in Anabaena (27) and fatty acid desaturases in Synechococcus (25) and Synechocystis (15). In these cold shock-induced cyanobacterial gene families, as with the crh gene family in Anabaena, regulation of expression is gene specific. The presence or absence of crhC transcripts is similar to that observed for desB (15, 25), while the constitutive but cold-enhanced expression of crhB is similar to that of desA (15, 25) and rbpB and rbpC (27).

The characteristics of the crhC promoter and crhC expression are consistent with a class I cold shock gene (31), while the cold shock enhancement of crhB expression implies that it is a class II cold shock gene. In this scenario, CrhC and CrhB would perform distinct roles in the acclimation of cyanobacteria to the cold shock state. These roles could involve an interaction of the RNA helicases with cold-induced RNA substrates, the ribosome, and/or accessory proteins required for cold acclimation. Specifically, RNA helicases could remove cold-stabilized secondary structures in cold shock mRNAs, thereby overcoming the cold-induced blockage of translation initiation under cold shock conditions (31).

In conclusion, our results indicate that the RNA helicase genes characterized in this report are differentially expressed in Anabaena. While crhB expression is enhanced in the cold, crhC expression is limited specifically to cold shock conditions. This implies that both RNA helicases are involved in the cold acclimation process in cyanobacteria; however, they appear to perform different roles in this adaptation. Division of labor between RNA helicases in a model prokaryotic organism like a cyanobacterium will provide an ideal system in which to study the factors controlling the expression of and the physiological functions(s) performed by RNA helicases. We are currently investigating the physiological and enzymatic functions performed by the crh RNA helicase genes in Anabaena.

ACKNOWLEDGMENTS

We are grateful to M. Pickard for providing Taq polymerase and A. Vioque for providing the Anabaena RNase P gene.

This work was supported by a Natural Sciences and Engineering Research Council (NSERC) of Canada postgraduate scholarship to W.C.M. and an NSERC operating grant to G.W.O.

REFERENCES

  • 1.Brander K A, Mandel T, Owttrim G W, Kuhlemeier C. Highly conserved genes coding for eukaryotic translation initiation factor eIF-4A of tobacco have specific alterations in functional motifs. Biochim Biophys Acta. 1995;1261:442–444. doi: 10.1016/0167-4781(95)00052-i. [DOI] [PubMed] [Google Scholar]
  • 2.Cho H-S, Ha N-C, Kang L-W, Chung K M, Back S H, Jang S K, Oh B-H. Crystal structure of RNA helicase from genotype 1b hepatitis C virus: a feasible mechanism of unwinding duplex RNA. J Biol Chem. 1998;273:15045–15052. doi: 10.1074/jbc.273.24.15045. [DOI] [PubMed] [Google Scholar]
  • 3.Curtis S E, Martin J A. The transcriptional apparatus and the regulation of transcription initiation. In: Bryant D A, editor. The molecular biology of cyanobacteria. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1994. pp. 613–639. [Google Scholar]
  • 4.Fang L, Jiang W, Bae W, Inouye M. Promoter-independent cold-shock induction of cspA and its derepression at 37°C by mRNA stabilization. Mol Microbiol. 1997;23:355–364. doi: 10.1046/j.1365-2958.1997.2351592.x. [DOI] [PubMed] [Google Scholar]
  • 5.Fuller-Pace F V. RNA helicases: modulators of RNA structure. Trends Cell Biol. 1994;4:271–274. doi: 10.1016/0962-8924(94)90210-0. [DOI] [PubMed] [Google Scholar]
  • 6.Gorbalenya A E, Koonin E V. Helicases: amino acid sequence comparisons and structure-function relationships. Curr Opin Struct Biol. 1993;3:419–429. [Google Scholar]
  • 7.Gray M W, Doolittle W F. Has the endosymbiont hypothesis been proven? Microbiol Rev. 1982;46:1–42. doi: 10.1128/mr.46.1.1-42.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Jiang W, Fang L, Inouye M. The role of the 5′-end untranslated region of the mRNA for CspA, the major cold-shock protein of Escherichia coli, in cold-shock adaptation. J Bacteriol. 1996;178:4919–4925. doi: 10.1128/jb.178.16.4919-4925.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Jones P G, Mitta M, Kim Y, Jaing W, Inouye M. Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli. Proc Natl Acad Sci USA. 1996;93:76–80. doi: 10.1073/pnas.93.1.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Kallas T, Rebiere M-C, Rippka R, Tandeau de Marsac N. The structural nif genes of the cyanobacterium Gloeothece sp. and Calothrix sp. share homology with those of Anabaena sp., but the Gloeothece genes have a different arrangement. J Bacteriol. 1983;155:427–431. doi: 10.1128/jb.155.1.427-431.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Kalman M, Murphy H, Cashel M. rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote. New Biol. 1991;3:886–895. [PubMed] [Google Scholar]
  • 12.Kaneko T, Tanaka A, Sato S, Kotani H, Sazuka T, Miyajima N, Sugiura M, Tabata S. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome. DNA Res. 1995;2:153–166. doi: 10.1093/dnares/2.4.153. [DOI] [PubMed] [Google Scholar]
  • 13.Kujat, S. L., and G. W. Owttrim. Expression of a cyanobacterial RNA helicase is regulated by light via cellular redox state. Submitted for publication.
  • 14.Ligon P J, Meyer K G, Martin J A, Curtis S E. Nucleotide sequence of a 16S rRNA gene from Anabaena sp. strain PCC 7120. Nucleic Acids Res. 1991;19:4553. doi: 10.1093/nar/19.16.4553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Los D A, Ray M K, Murata N. Differences in the control of the temperature-dependent expression of four genes for desaturases in Synechocystis sp. PCC 6803. Mol Microbiol. 1997;25:1167–1175. doi: 10.1046/j.1365-2958.1997.5641912.x. [DOI] [PubMed] [Google Scholar]
  • 16.Mitta M, Fang L, Inouye M. Deletion analysis of cspA of Escherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction. Mol Microbiol. 1997;26:321–335. doi: 10.1046/j.1365-2958.1997.5771943.x. [DOI] [PubMed] [Google Scholar]
  • 17.Nicol S M, Fuller-Pace F V. The “DEAD box” protein DbpA interacts specifically with the peptidyltransferase center in 23S rRNA. Proc Natl Acad Sci USA. 1995;92:11681–11685. doi: 10.1073/pnas.92.25.11681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Nishi K, Morel-Deville F, Hershey J W B, Leighton T, Schnier J A. An eIF-4A-like protein is a suppressor of an Escherichia coli mutant defective in 50S ribosomal subunit assembly. Nature. 1988;336:496–498. doi: 10.1038/336496a0. [DOI] [PubMed] [Google Scholar]
  • 19.Ohmori H. Structural analysis of the rhlE gene of Escherichia coli. Jpn J Genet. 1994;69:1–12. doi: 10.1266/jjg.69.1. [DOI] [PubMed] [Google Scholar]
  • 20.Owttrim G W, Hofmann S, Kuhlemeier C. Divergent genes from translation initiation factor eIF-4A are coordinately expressed in tobacco. Nucleic Acids Res. 1991;19:5491–5496. doi: 10.1093/nar/19.20.5491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Owttrim G W, Mandel T, Trachsel H, Thomas A A M, Kuhlemeier C. Characterization of the tobacco eIF-4A gene family. Plant Mol Biol. 1994;26:1747–1757. doi: 10.1007/BF00019489. [DOI] [PubMed] [Google Scholar]
  • 22.Pause A, Sonenberg N. Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A. EMBO J. 1992;11:2643–2654. doi: 10.1002/j.1460-2075.1992.tb05330.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Py B, Higgins C F, Krisch H M, Carpousis A J. A DEAD-box RNA helicase in the Escherichia coli RNA degradosome. Nature. 1996;381:169–172. doi: 10.1038/381169a0. [DOI] [PubMed] [Google Scholar]
  • 24.Richardson J P, Greenblatt J. Control of RNA chain elongation and termination. In: Neidhardt F C, Curtiss III R, Ingraham J L, Lin E C C, Low K B, Magasanik B, Reznikoff W S, Riley M, Schaechter M, Umbarger H E, editors. Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Washington, D.C: ASM Press; 1996. pp. 822–848. [Google Scholar]
  • 25.Sakamoto T, Bryant D A. Temperature-regulated mRNA accumulation and stabilization for fatty acid desaturase genes in the cyanobacterium Synechococcus sp. strain PCC 7002. Mol Microbiol. 1997;23:1281–1292. doi: 10.1046/j.1365-2958.1997.3071676.x. [DOI] [PubMed] [Google Scholar]
  • 26.Sambrook J, Fritsch E F, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press; 1989. [Google Scholar]
  • 27.Sato N. A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena sp. strain M3. Nucleic Acids Res. 1995;23:2161–2167. doi: 10.1093/nar/23.12.2161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Schmid S R, Linder P. D-E-A-D protein family of putative RNA helicases. Mol Microbiol. 1992;6:283–292. doi: 10.1111/j.1365-2958.1992.tb01470.x. [DOI] [PubMed] [Google Scholar]
  • 29.Stanier R Y, Cohen-Bazire G. Phototrophic prokaryotes: the cyanobacteria. Annu Rev Microbiol. 1977;31:225–274. doi: 10.1146/annurev.mi.31.100177.001301. [DOI] [PubMed] [Google Scholar]
  • 30.Stein J L, Marsh T L, Wu K Y, Shizuya H, DeLong E F. Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon. J Bacteriol. 1996;178:591–599. doi: 10.1128/jb.178.3.591-599.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Thieringer H A, Jones P G, Inouye M. Cold shock and adaptation. Bioessays. 1998;20:49–57. doi: 10.1002/(SICI)1521-1878(199801)20:1<49::AID-BIES8>3.0.CO;2-N. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES