Table 1.
Map and model building statistics for the IGF-I-complexed HybZipa
| Head | Legs | |
|---|---|---|
| PDB code | 7S0Q | 7S8V |
| Composition (#) | ||
| Chains | 3 | 2 |
| Atoms (including hydrogens) | 15,551 | 12,651 |
| Protein residues | 958 | 782 |
| Glycan residues | 16 | 12 |
| Bonds (RMSD) | ||
| Length (Å) (# > 4σ) | 0.003 (0) | 0.003 (0) |
| Angles (°) (# > 4σ) | 0.521 (0) | 0.590 (0) |
| MolProbity score | 2.07 | 1.74 |
| Clash score | 9.97 | 5.45 |
| Ramachandran plot (%) | ||
| Outliers/allowed/favored | 0.00/9.92/90.08 | 0.00/7.03/92.97 |
| Rotamer outliers (%) | 0.00 | 0.14 |
| Cβ outliers (%) | 0.00 | 0.00 |
| Peptide plane (%) | ||
| Cis proline/general | 2.4/0.00 | 1.9/0.0 |
| Twisted proline/general | 0.0/0.0 | 0.0/0.1 |
| CαBLAM outliers (%) | 4.80 | 3.05 |
| ADP | ||
| Iso/aniso (# atoms) | 7,891/0 | 6,457/0 |
| Protein (min/max/mean) | 69.99/206.73/118.92 | 112.49/458.95/186.24 |
| Glycan (min/max/mean) | 93.63/190.15/135.24 | 124.02/182.08/158.68 |
| Occupancy (# atoms) | ||
| Occ = 1/0.5 /0.0 | 15,551/0/0 | 12,651/0/0 |
| Map | ||
| Resolution (Å): FSC independent half maps | 3.70 | 3.73 |
| Local resolution range (Å) | 2.8–8.0 | 2.8–8.0 |
| Sharpening B factor (Å2) | -35.7 | -45.7 |
| Model versus map | ||
| CCmask | 0.71 | 0.65 |
| CCbox | 0.82 | 0.68 |
| CCvolume | 0.71 | 0.65 |
| CCindividual chains | ||
| HybZip IGF-1R/IR | 0.77/0.72 | 0.68/0.70 |
| IGF-I | 0.73 | N/A |
| Glycan IGF-1R/IR | 0.68/0.71 | 0.58/0.62 |
| Resolution (Å): FSC, masked map versus model at 0.143 | 3.64 | 3.80 |
RMSD, root-mean-square deviation; N/A, not applicable.
a
See also Figures S3 and S4.