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. 2022 Aug 4;11(15):2404. doi: 10.3390/cells11152404

Figure 3.

Figure 3

RNF20/40 and H2BK120ub facilitates transcriptional elongation. (A) To initiate H2B ubiquitination, RNF20/40, an E2 enzyme (UBE2A/B), and PAF1 create a complex that links to RNA polymerase II (Pol II) through WAC, following CDK9-dependent RNA Pol II phosphorylation. (B) H2BK120ub promotes FACT accumulation via interactions with the Spt16 subunit of FACT complex. In turn, the activity of FACT enables RNA Pol II transcriptional elongation by displacing the H2A/H2B dimer from the nucleosome. Subsequently, H2BK120ub and Spt16 also cooperate to promotes nucleosome reassembly and stabilization.