Figure 3.

Heterodimerization does not affect basal Ca²⁺ levels in N1E-115 cells. Neuroblastoma cells were transfected with Ca²⁺ indicator GCaMP6f, either alone or together with 5-HT_2A-mTurquoise2, TrkB-YPet, or both. Basal Ca²⁺ activity was calculated as F/F_max. To saturate Ca²⁺ levels, 10 µM ionomycin was applied after 6 min of recording. The saturated Ca²⁺ signal was used as F_max. Data are presented as means ± SEM (12 ≤ n ≤ 17). *** p < 0.001 (Kruskal–Wallis test with Dunn’s multiple-comparison post hoc test).