Table 4. Composition of Panel Two.
This panel was developed to sort CD73 + CD90 + and podoplanin + CD36 + cells from placental villous core using a BD FACS Aria II.
| Antibody | Fluorophore | Clone | Dose (μL) | Supplier |
|---|---|---|---|---|
| CD45 | FITC | HI30 | 0.3 | BD |
| PDPN | PE | NC-08 | 0.3 | Biolegend |
| CD26 | PE/Cy7 | BA5b | 0.6 | BD |
| CD271 | PE/Dazzle 594 | C40-1457 | 0.6 | BD |
| CD144 | PerCP-5.5 | 55–7 H1 | 0.6 | BD |
| CD90 | Alexa700 | 5E10 | 0.6 | Biolegend |
| CD36 | APC-Cy7 | 5–271 | 0.6 | Biolegend |
| β4 integrin | APC | 450-9D | 0.6 | Thermofisher |
| CD31 | BV480 | WM59 | 0.3 | BD |
| CD73 | BV785 | AD2 | 0.3 | BD |
| CD34 | BUV395 | 581 | 1.25 | BD |