A) and C) Representative double immunofluorescence microscopy (IFM) images of ileal, and colonic tissue double stained with antibodies against MUC2 (red channel) and KLF4 (green channel, white arrows). Magnification: ×100 (inset 200X), scale bar = 100 μm; B) and D) Quantification of ileal and colonic IFM optical intensity for MUC2 (red bars) and KLF4 (green bars). All data error bars represent ±SD, with **p < 0.01, ****p < 0.0001 and ns = not significant.