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. Author manuscript; available in PMC: 2022 Aug 17.
Published in final edited form as: Science. 2022 Feb 3;375(6580):515–522. doi: 10.1126/science.abe7489

Fig. 1. Overview of 6mASCOPE for quantitative 6mA deconvolution.

Fig. 1.

(A) Reference-free 6mA analysis of single molecules. Each molecule (short insert) is sequenced for a large number of passes (subreads). The subreads are combined to a circular consensus sequence (CCS), serving as the molecule-specific reference for in silico IPD estimation, and provide repeated measures of IPD values for 6mA analysis (Methods). Blue segment: SMRT adapter. (B) After single molecule 6mA analysis (a red dot indicates a 6mA event), CCSs (black rods) from a sequenced gDNA sample are separated into the eukaryotic genome (green) and contamination sources (blue and yellow). The 6mA/A levels of each species (or genomic region) are estimated using a machine learning model trained across a wide range of 6mA abundance, with defined confidence intervals.