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. 2022 Jul 14;149(14):dev200154. doi: 10.1242/dev.200154

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Progressive segregation of IFE-resident cell types during postnatal tail skin development. (A) Immunostaining of tail epidermis wholemounts from wild-type C57BL/6 mice for Trp2, K31 and LC markers MHCII (P5, P10) or langerin (P21). Representative for n=3. (B) Immunostaining of tail epidermis wholemounts from wild-type C57BL/6 mice for K31 and γδTCR. Representative for n=3. (C) Quantification of A: MC density (MC number/mm²) in tail epidermis. n=3; ns, P=0.2458 (P5 versus P10); ns, P=0.4338 (P5 versus P21); ns, P=0.8820 (P10 versus P21); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. (D) Quantification of A: LC density (LC number/mm²) in tail epidermis. n=3; ns, P=0.9750 (P5 versus P10); **P=0.0031 (P5 versus P21); **P=0.0038 (P10 versus P21); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. (E) Quantification of B: DETC density (DETC number/mm²) in tail epidermis. n=3; ns, P=0.2455 (P5 versus P10); ns, P=0.2082 (P5 versus P21); ns, P=0.9905 (P10 versus P21); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. (F) Quantification of A and B: scale:interscale unit size (mm²). n=6; ns, P=0.0523 (P5 versus P10); ****P<0.0001 (P5 versus P21); ****P<0.0001 (P10 versus P21); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. is, interscale; ns, not significant; s, scale. Scale bars: 75 µm (A); 100 µm (B).