Extended Data Fig. 5. Efficient target cleavage by MIWI guided by endogenous piRNAs requires GTSF1.

Representative target cleavage assay using MIWI piRISC programmed with an abundant mouse pachytene piRNA antisense to the L1MC transposon (left) or targeting the Scpep1 mRNA (right). piRNA sequences are in orange; below each piRNA sequence, the target in the RNA sequence is in black. Scissors indicates the cleavage site of the target RNA. The last lane in each gel shows the extent of cleavage using the reciprocal, non-cognate target, incubated with piRISC for 240 min as a negative control. Where data points from the two trials overlap, jitter has been introduced for clarity. Curves show the fit of the burst-and-steady equation to the mean of the two trials. For gel source data, see Supplementary Fig. 1.