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. 2022 Jun 30;608(7923):618–625. doi: 10.1038/s41586-022-05009-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, MIWI piRISC target cleavage in the presence of GTSF1 for targets with varying complementarity to synthetic piRNA guide. Top, multiple-turnover conditions; bottom, single-turnover conditions. All reactions contained saturating amounts of GTSF1. b, Target-cleavage assay using targets complementary to piRNA guide nucleotides g2–g16 or g2–g30 and MIWI or MILI loaded with piRNAs of the indicated lengths, with or without GTSF1 (mean ± s.d., n = 3). c, Absolute and relative pre-steady-state and steady-state rates of cleavage of the g2–g30 target by MIWI loaded with piRNAs of the indicated lengths in the presence of GTSF1 (mean ± s.d., n = 3), E_apparent, apparent active enzyme concentration estimated from fitting data to equation (1). For gel source data, see Supplementary Fig. 1.