Table 4. Kinetics of excision of chain terminating cytidine analogs by ExoN.

Data were fit to a single exponential to determine the observed rate of cleavage and half-life (t_1/2).

	− SARS replicasea		+ SARS replicaseb
NTP	rate (min−1)	t1/2 (min)	rate (min−1)	t1/2 (min)
CTP	0.23 ± 0.01	3	0.0027 ± 0.0004	260
UTP	-	-	0.0046 ± 0.0002	150
3ʹ-dCTP	0.014 ± 0.002	50	0.0032 ± 0.0004	220
ddhCTP	0.089 ± 0.01	8	0.0018 ± 0.0002	390
2’-C-Me-CTP	0.15 ± 0.02	5	0.0028 ± 0.0001	250

^aExperiment shown in Fig. 9.

^bExperiment shown in Fig. 10.