Table 5.

Subtilisin irritation potential in the EpiSkin Reconstructed Human Epidermis Model. Mean OD570 Values and Viabilities for the Negative Control Item (Dulbecco’s Phosphate Buffered Saline (DPBS)), Positive Control Item (Sodium dodecyl sulphate (SDS), prepared as a 5% w/v aqueous solution) and Test Item. N = 3.

Item	OD570 of tissues	Mean OD570 of triplicate tissues	± SD of OD570	Relative individual tissue viability (%)	Relative mean viability (%)	± SD of Relative mean viability (%)
Negative Control Item	0.705	0.718	0.096	98.2	100	13.4
	0.629			87.6
	0.82			114.2
Positive Control Item	0.212	0.148	0.06	29.5	20.6	8.3
	0.14			19.5
	0.093			13.0
Subtilisin	0.465	0.563	0.098	64.8	78.4	13.7
	0.564			78.6
	0.661			92.1