Table 6.
Bacterial reverse mutation assay; data from experiment 2 only. Mean number of revertant colonies per plate and fold increase relative to vehicle (in parentheses). Abbreviations: TOS: Total Organic Solids, NaN3: Sodium azide, 2-NF: 2-Nitrofluorene, NQO: 4-Nitroquinoline-1-oxide, AAC: 9-Aminoacridine, B[a]P: Benzo[a]pyrene, AAN: 2-Aminoanthracene.
| Compound | μg TOS/mL | S9 | TA98 | TA100 | TA1535 | TA1537 | WP2 uvrA pKM101 |
|---|---|---|---|---|---|---|---|
| Purified water | – | − | 40.7 | 112.3 | 13.3 | 14.3 | 112.3 |
| Subtilisin | 50 | − | 39.7 (1.0) | 105.0 (0.9) | 10.7 (0.8) | 13.0 (0.9) | 116.3 (1.0) |
| Subtilisin | 150 | − | 47.3 (1.2) | 117.0 (1.0) | 10.3 (0.8) | 11.0 (0.8) | 119.0 (1.1) |
| Subtilisin | 500 | − | 40.0 (1.0) | 126.3 (1.1) | 12.7 (0.9) | 17.7 (1.2) | 113.7 (1.0) |
| Subtilisin | 1500 | − | 41.3 (1.0) | 128.3 (1.1) | 13.7 (1.0) | 12.3 (0.9) | 147.3 (1.3) |
| Subtilisin | 5000 | − | 40.0 (1.0) | 128.7 (1.1) | 17.7 (1.3) | 11.3 (0.8) | 150.7 (1.3) |
| 2NF | 1.25 | − | 100.7 (2.5) | ||||
| NQO | 0.3 | − | 597 (5.3) | ||||
| NaN3 | 1.9 | − | 111.7 (8.4) | ||||
| AAC | 31.3 | − | 58.7 (4.1) | ||||
| NQO | 0.3 | − | 2560 (22.8) | ||||
| Purified water | – | + | 47.3 | 107.3 | 10.7 | 12.7 | 150.3 |
| Subtilisin | 50 | + | 29.7 (0.6) | 114.0 (1.1) | 17.3 (1.6) | 7.3 (0.6) | 178.7 (1.2) |
| Subtilisin | 150 | + | 39.0 (0.8) | 115.7 (1.1) | 17.7 (1.7) | 11.7 (0.9) | 178.0 (1.2) |
| Subtilisin | 500 | + | 32.3 (0.7) | 118.7 (1.1) | 19.3 (1.8) | 9.7 (0.8) | 161.0 (1.1) |
| Subtilisin | 1500 | + | 41.7 (0.9) | 126.0 (1.2) | 17.0 (1.6) | 9.7 (0.8) | 182.3 (1.2) |
| Subtilisin | 5000 | + | 44.3 (0.9) | 109.3 (1.0) | 14.7 (1.4) | 13.7 (1.1) | 178.3 (1.2) |
| B[a]P | 9.4 | + | 134.7 (2.8) | ||||
| AAN | 6.3 | + | 677.7 (6.3) | 160.3 (15) | |||
| AAN | 9.4 | + | 106 (8.4) | 447.3 (3.0) |