Correction: Mol Cancer 20, 51 (2021)
https://doi.org/10.1186/s12943-021-01333-7
In our BMC Research publication in Molecular Cancer entitled ‘CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing ’[1], we misplaced two images in Fig. 2C and Fig.S3D. These errors inadvertently happened during the stage of our figure assembly with photoshop software. The corrected version of Fig. 2 and Fig.S3 has been provided.
Fig 2.
CircNEIL3 promotes the proliferation, migration and invasion of PDAC cells in vitro. a. RT-qPCR analysis of circNEIL3 and NEIL3 mRNA expression in CFPAC-1 and MiaPaca-2 cells transfected with a lentivirus and circNEIL3 plasmid. b. The growth curves of cells were evaluated by CCK-8 assays after knocking down and overexpressing circNEIL3 in CFPAC-1 and MiaPaca-2 cells. c-d. Colony formation assays were performed to evaluate cell proliferation. e-f. EdU assays of PDAC cells was performed to evaluate cell proliferation. The samples were imaged at 200× magnification. Scale bar = 50 μm. g-h. Transwell assays were performed to assess the migration and invasion abilities of PDAC cells. The samples were imaged at 100× magnification. Scale bar = 100 μm. i-j. Cell migration was assessed using a wound healing assay. The samples were imaged at 100× magnification. Scale bar = 100 μm. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001
The correction does not affect the conclusion or discussion of this article.
Supplementary Information
Additional file 1: Figure S3. ADAR1 downregulation reverses the oncogenic phenotype induced by circNEIL3 overexpression. a-i. CCK-8, colony formation, EdU, transwell and wound healing assay results showed that transfection with the ADAR1 shRNA inhibited the proliferation, migration, and invasion abilities of MiaPaca-2 cells, which was reversed after cotransfection with the circNEIL3 plasmid. The EdU samples were imaged at 200× magnification. Scale bar = 50 μm. The transwell and hound healing samples were imaged at 100× magnification. Scale bar = 100 μm. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Footnotes
Peng Shen, Taoyue Yang, Qun Chen and Hao Yuan contributed equally to this work.
Contributor Information
Zipeng Lu, Email: surgeonmark@hotmail.com.
Kuirong Jiang, Email: jiangkuirong@njmu.edu.cn.
Reference
- 1.Shen P, Yang T, Chen Q, et al. CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing. Mol Cancer. 2021;20:51. doi: 10.1186/s12943-021-01333-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
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Supplementary Materials
Additional file 1: Figure S3. ADAR1 downregulation reverses the oncogenic phenotype induced by circNEIL3 overexpression. a-i. CCK-8, colony formation, EdU, transwell and wound healing assay results showed that transfection with the ADAR1 shRNA inhibited the proliferation, migration, and invasion abilities of MiaPaca-2 cells, which was reversed after cotransfection with the circNEIL3 plasmid. The EdU samples were imaged at 200× magnification. Scale bar = 50 μm. The transwell and hound healing samples were imaged at 100× magnification. Scale bar = 100 μm. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.