Fig. 5.

Performance of ZicoSeq when the sequencing depth differs by fourfold between the groups. The results are based on stool data under the balanced setting (sample size = 100, taxa number = 500). Performance is assessed by the observed false discovery rate (FDR) level and average true positive rate (TPR) in comparison with all evaluated methods. The color of the bar indicates the FDR control performance. The blue color indicates that the method controls the FDR at the 5% target level (the 95% confidence interval covers 5%). Yellow, red, and gray colors indicate the observed FDR level in (0.05–0.1), (0.1, 0.2), and (0.2, 1), respectively. The length of the bar is proportional to the TPR, and the actual TPR is also shown in the bar. FDR and TPR ranks are based on the average FDR and TPR score across signal densities and differential modes. The order of the method is arranged based on the sum of the FDR and TPR ranks