Physical function, body mass index, and fitness outcomes in children, adolescents, and emerging adults with craniopharyngioma from proton therapy through five years of follow-up

Abstract

Purpose

Children diagnosed with craniopharyngioma are vulnerable to adverse health outcomes. Characterization of body mass index (BMI), physical function, and cardiopulmonary fitness in those treated with proton radiotherapy (PRT) will serve to design interventions to improve outcomes.

Methods

Ninety-four children with craniopharyngioma completed physical function testing prior to PRT and annually for 5 years. For each outcome, age- and sex-specific z-scores were calculated using normative values. Participants with z-scores > 1.5 or < − 1.5 were classified as impaired. Those with z-scores > 2.0 or < − 2.0 were classified as significantly impaired. Descriptive statistics were used to describe study outcomes and change in prevalence of impairments from 2 to 5 years after treatment.

Results

Nearly half of participants [45.2%, 95% confidence interval (CI) 39.4, 51.0] had mean BMI z-scores > 1.5 at baseline, with prevalence increasing to 66.7% (95% CI 61.5, 71.9) at 5 years. More than half of participants (54.2%, 95% CI 48.4, 60.0) had knee extension strength z-scores < − 1.5 at baseline, with prevalence increasing to 81.3% (95% CI 77.7, 84.9) at 5 years. BMI and knee extension strength had the largest proportion of participants impaired at both 2 and 5 years (53.2% and 62.3%, respectively). Resting heart rate had the highest proportion of participants not impaired at 2 years but became impaired at 5 years (26.6%).

Conclusions

Children with craniopharyngioma have BMI and fitness abnormalities at diagnosis and continue 5 years after treatment. This cohort may benefit from interventions designed to improve BMI, strength, and resting indicators of cardiopulmonary fitness.

Supplementary Information

The online version contains supplementary material available at 10.1007/s11060-022-04116-2.

Introduction

Children diagnosed with craniopharyngioma have a high rate of survival, with over 95% surviving 5 years or longer [1]. The sellar/suprasellar location of this tumor and current treatments leave survivors vulnerable to a spectrum of adverse outcomes including body mass index (BMI) [2] and fitness impairments [3, 4]. Hormone deficiencies [2] and metabolic disorders [2] are common. While replacement therapy can normalize hormonal function, these children have persistent problems with obesity. Weight gain is both organic [5] and behavioral [6], manifested as elevated BMI, increased waist circumference, and an abnormal waist to height ratio [7]. In long-term survivors of childhood cancer, particularly among those with untreated growth hormone deficiency, BMI abnormalities are associated with muscle weakness and suboptimal cardiorespiratory fitness [8].

Obesity, weakness, and poor cardiorespiratory fitness during childhood are a problematic constellation of impairments, predisposing individuals to long-term chronic physical and psychosocial health conditions which are associated with poor adaptive physical function in the general population [9, 10]. These are major concerns in long-term survivors of childhood craniopharyngioma [11, 12]. Previous studies among children with craniopharyngioma are limited to obesity rates [13], cross-sectional methodology [4], or description of cohorts treated with conventional photon-based radiation therapy [14]. Because most children diagnosed with craniopharyngioma are now treated with proton radiotherapy (PRT), a modality designed to reduce late sequalae by limiting radiation exposure in healthy tissue [15], fewer or less severe sequelae are expected.

Hypothalamic function influences BMI and hunger through hormonal regulation of insulin, leptin and ghrelin [13], and cardiorespiratory fitness, by regulation of the parasympathetic and sympathetic nervous activity [16]. Sparing tissue not involved with the tumor could help improve these outcomes. Limited, but affirmative results among children with other brain tumor histologies indicate reduced doses of radiation to the hypothalamus result in better endocrine outcomes with PRT.¹⁰ To our knowledge, no study has characterized BMI, muscle strength, and cardiorespiratory fitness among children with craniopharyngioma treated with PRT. Our goal was to describe the proportion of participants that have impaired BMI, muscle strength, adaptive physical function, and cardiorespiratory fitness, as well as describe the proportion of participants that change or maintain their impairment status between 2 and 5 years after treatment. The findings from this study will inform monitoring guidelines, caregiver education, and development of targeted interventions.

Methods

Participants

Participants were enrolled on the RT2CR protocol (NCT01419067) at St. Jude Children’s Research Hospital (SJCRH, n = 72) or the University of Florida Health Proton Therapy Institute (UFHPTI, n = 22) between 8/22/2011 and 1/19/2016. The median age at enrollment was 9.21 years (range 0.88–20.13 years). Following baseline assessments performed at SJCRH, participants were treated with PRT at UFHPTI using passive scattering methods and 0.5 cm clinical target volume margin. The prescribed dose was 54 Gy (RBE) in 30 fractions. Surgery prior to PRT was individualized and performed at referring centers or enrollment sites. Upon completion of PRT, patients were followed at SJCRH at regular intervals (± 3 months) which included 5 years of annual assessments and measures contained in this report. Written informed consent was obtained from all participants and the protocol was approved by the SJCRH Institutional Review Board. All performance assessments were completed by master’s level, American College of Sports Medicine certified Exercise Specialists using standardized instructions. All staff participate in yearly inter-rater reliability and are trained to encourage participants during effort-dependent tests.

Demographic and clinical characteristics

Demographic and clinical characteristics were recorded for each participant and included sex, race, and surgical approach (craniotomy vs. no craniotomy). Surgical approach was dichotomized into two groups (craniotomy and no craniotomy). Patients who received one or two craniotomies, or multiple approaches that included a craniotomy were categorized in the craniotomy group. Patients who received an endoscopic resection, ommaya-closed, ommaya-open, a transsphenoidal, or had no surgery were categorized in the no craniotomy group. Participants were determined to have diabetes insipidus if they required permanent treatment with desmopressin prior to baseline assessment [17].

Anthropometrics

Height and weight were measured without shoes using a wall mounted stadiometer (SECA, Hanover, MD, USA) in centimeters (cm), and on an electronic scale (Scale-tronix, White Plains, NY, USA) in kilograms (kg), respectively. BMI was calculated as weight in kg divided by height in meters squared. Sex- and age-adjusted BMI z-scores were calculated using the 2000 Center of Disease Control (CDC) growth charts [18]. Waist circumference was measured using a Gulick tape measure at the smallest point between the xyphoid process and the umbilicus. The waist to height ratio (WHR) was calculated by dividing waist circumference by height.

Flexibility and strength

Low back and hamstring flexibility was assessed using a Flex-Tester sit and reach box (Novel Products, Inc., Rockton, IL, USA). Participants sat with their hips at ninety degrees and their knees extended. Bending only at their hips, participants reached forward as far as possible. The test was repeated, and the best distance to the 0.5 cm was recorded [19]. Hand grip strength was assessed using a Jamar hand grip dynamometer (Performance Health, Warrenville, IL, USA). With the elbow in ninety degrees of flexion and the wrist and shoulder in neutral, participants squeezed until peak force was reached. The better of two trials for each hand was documented in kg [20]. Knee extension strength was measured with a Biodex System IV Dynamometer (Biodex Medical Systems, Shirley, New York City, NY, USA). Participants sat in a supported position with the hips at 95 degrees of flexion and the knees in 60 degrees of flexion. Three trials of maximum isometric strength, alternating extension and flexion, were performed for 5 s each, with 5 s of rest in between. Peak isometric extension normalized for body weight for each leg was recorded in newtons (N)/kg [21].

Balance

Balance was measured using the Sensory Organization Test (SOT; Neurocom Smart Equitest, Natus Medical Inc., Pleasanton, CA, USA). Participants stood upright on a force plate with a visual surround, and experienced six 20 s perturbations during which the percentage of time spent inside a 12.5 degree sway envelope was recorded [22]. These included (1) eyes open, (2) eyes closed, (3) eyes open—vision sway referenced (the visual surround moved in concert with anterior–posterior sway), (4) eyes open—proprioception sway referenced (the force plate moved in concert with anterior–posterior sway), (5) eyes closed—proprioception sway referenced, and (6) eyes open—vision and proprioception sway referenced. The composite score (out of 100 possible) for all six conditions was used for analysis.

Adaptive physical function

Gross and fine motor skills were assessed using the Bruininks-Oseretsky Test of Motor Proficiency 2nd Edition Short Form (BOT-SF) [23]. This standardized subset of tests include fine motor precision, fine motor integration, manual dexterity, bilateral coordination, balance, running speed and agility, upper-limb coordination, and strength. Raw scores are converted to a standard score based on age, sex, and the type of pushup performed (knee or full pushup). Standard scores have a mean of 50 and a SD of 10. The BOT-SF has excellent test–retest reliability (intraclass correlation coefficient [ICC] = 0.80–0.99) [24].

Metabolic and cardiopulmonary exercise testing

Resting metabolic rate (RMR) was measured via indirect calorimetry (UltimaCardio2; MGC Diagnostics, St. Paul, MN, USA). Participants fasted for 8 h prior to assessment, and rested supine for 20 min, wearing a face mask attached to a low flow pneumotach. The RMR [kilocalories (kcals)] was calculated using the modified Weir equation [25]. This test was performed on a separate day from all other performance testing.

A ramping maximal cardiopulmonary exercise test was also completed using a modified Balke treadmill protocol [26]. Participants walked 3–3.5 miles per hour, depending on ability, starting at 0% incline. Every minute, the incline increased by 1% until the patient reached maximal exertion. While the treadmill was the preferred method of testing, some participants were tested with a leg ergometer using a ten watt per minute ramping protocol because of small stature, younger age, or balance deficits. Participants wore a 12-lead electrocardiogram, automated blood pressure (BP) monitor, and a pulse oximeter for the duration of the test and into recovery. The BP and heart rate (HR) were recorded pre-test, at three-minute intervals during the test, immediately post-test, and into recovery. Breath-by-breath gas exchange was evaluated continuously, using the mid 5 of 7 breaths, to assess peak oxygen uptake (peak volume of oxygen; peak VO₂; milliliters/kg/min) [27, 28]. The test was stopped at the request of the participant, at maximal exertion, or for any safety concerns.

Statistical analysis

Descriptive statistics were used to characterize study participants. For each outcome, age and sex specific z-scores were calculated using established normative values. Participants with z-scores < − 1.5 (or > 1.5 for WHR, BMI, resting systolic BP, resting HR, and peak VO₂ were classified as impaired; participants with z-scores < − 2.0 (or > 2.0 for the measures listed above) were classified as significantly impaired). Missing outcome data from each functional test were reviewed by two authors (RP, MW); those whose medical status precluded test completion were also classified as impaired. All other missing outcome data were omitted from analyses. To compare change in impairment status after conclusion of treatment, each outcome was classified as impaired or not impaired at 2 and 5 years and compared as the following: not impaired at 2 or 5 years, not impaired at 2 but impaired at 5 years, impaired at 2 and 5 years, or impaired at 2 but not impaired at 5 years. A sensitivity analysis examined the impact of excluding participants who started treatment over 1 year after diagnosis (n = 22) and those who did not complete all timepoints. A second sensitivity analysis was done using z-scores that were calculated using means that were adjusted for hypothyroid medication (yes or no), growth hormone (yes or no) and corticosteroids (yea or no). Finally, a supplemental analysis was done calculating the z-scores of those in the craniotomy surgery group and no craniotomy group separately. Data were analyzed with SAS version 9.4 (SAS institute, Cary NC, USA).”

Results

Participants

The characteristics of participants are described in Table 1. The mean age at diagnosis was 9.0 (SD 4.52), 55.3% were female, and 64.9% were white. More than half of participants (61.7%) had a craniotomy. Tanner stages for participants in each time point are presented in (Online Resource Table 10). The sensitivity analysis, which removed patients who had treatment more than 1 year after diagnosis, did not substantially change the characteristics of the cohort (Online Resource Table 1).

Table 1.

Demographics and clinical characteristics

	Participants (n = 94)
Sex, n (%)
Female	51 (54.26%)
Male	43 (45.74%)
Race, n (%)
White	61 (64.89%)
Other	33 (35.11%)
Age at diagnosis, mean (SD)	9.0 (4.52)
Surgical approach, n (%)
Craniotomy	58 (61.70%)
No craniotomy	36 (38.30%)
CSF shunt placed, n (%)
Yes	32 (34.04%)
No	62 (65.96%)
Diabetes insipidus, n (%)
Yes	56 (59.57%)
No	38 (40.43%)

SD standard deviation, n number; % percentage; SD standard deviation

Fitness impairments

Mean (± SD) age and sex specific z-scores for each outcome are shown in Table 2. Mean BMI was consistently elevated at each time point with z-scores ranging from 1.3±1.1 at baseline to 1.7±0.8 at 5 years. The WHR z-scores were also elevated throughout the study period (baseline, 1.2±1.4; 5 years, 1.7±1.7). Mean peak VO₂ was lower than expected at baseline (− 0.9±0.9) and improved over time but was still lower than expected at 5 years (− 0.5±0.9). On average, hand grip and knee extension strength were more impaired than all other fitness measures. Hand grip strength declined from baseline (− 1.6±1.8) to 6 months (− 1.9±1.8), recovered to baseline levels, but was still impaired at 5 years (− 1.4±1.6). Mean knee extension strength z-score was − 2.1±1.0 at baseline and declined to − 2.9±1.4 by 5 years. Adjusting the z-scores for medication use yielded similar results (Online Resource Table 2). Those who received at least one craniotomy had worse outcomes compared to those who did not (Online Resource Table 3a, b). Tables 3 and 4 show the percentage of participants with fitness impairments more than 1.5 SD (3) and 2.0 SD (4) below (or above, for WHR, BMI, resting systolic BP, and HR) age and sex specific normative values. Nearly half of participants (45.2%, 95% confidence internal [CI] 39.4, 51.0) had a BMI more than 1.5 SD above the mean at baseline and this frequency increased to 66.7% (95% CI 51.5, 71.9) by 5 years. The percentage of participants with resting systolic BP 1.5 SD above the mean decreased from baseline (50%, 95% CI 44.1, 55.9) to 5 years (34.6%, 95% CI 29.3, 39.9). The percentage of participants with resting HR more than 1.5 SD above the mean increased from baseline to 5 years (baseline, 26.2%, 95% CI 21.7, 30.7; 5 years, 43.2%, 95% CI 37.5, 48.9). Over half of participants (54.2%, 95% CI 48.4, 60.0) had impaired knee extension strength at 1.5 SD below the mean and this frequency increased to 81.3% (95% CI 77.7, 84.9) by 5 years. Trends in percentages (> 2 SD) were similar for more severe impairment for all measures.

Table 2.

Age- and sex-specific z-scores (Mean and SD) for physical fitness measures in participants with craniopharyngioma during a 5-year follow-up period

	Baseline (n = 84)		6-months (n = 73)		1 year (n = 76)		2 years (n = 89)		3 years (n = 91)		4 years (n = 83)		5 years (n = 81)
	Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD	Mean	SD
Anthropometrics
Waist to height ratio	1.2	1.4	1.5	1.4	1.4	1.6	1.3	1.5	1.4	1.5	1.6	1.5	1.7	1.7
Body mass index	1.3	1.1	1.5	0.9	1.5	1.0	1.5	0.9	1.6	0.9	1.7	0.8	1.7	0.8
Cardiopulmonary fitness
Resting systolic blood pressurea	0.9	5.9	1.5	5.9	2.3	4.7	1.1	4.6	1.1	4.7	0.8	4.6	1.1	4.2
Resting heart rate	0.8	1.6	0.6	1.7	0.8	1.8	0.8	1.7	0.8	1.8	1.0	1.9	1.2	1.6
Peak oxygen uptake	− 0.9	0.9	− 0.8	1.0	− 0.8	1.0	− 0.8	0.9	− 0.6	0.8	− 0.5	0.9	− 0.5	0.9
Metabolism
Resting metabolic rate	− 0.6	0.7	− 0.7	0.7	− 0.6	0.8	− 0.2	0.7	− 0.5	0.7	− 0.4	0.8	− 0.4	0.8
Flexibility
Sit and reach	-0.5	1.2	-0.3	1.0	-0.2	1.1	-0.2	1.2	-0.4	1.2	-0.6	1.2	-0.7	1.3
Dorsiflexion ROM	0.3	1.7	0.5	1.3	0.2	1.4	-0.1	1.7	-0.3	1.5	-0.5	1.6	-0.3	1.4
Muscular strength
Hand grip strength	− 1.6	1.8	− 1.9	1.8	− 1.6	1.6	− 1.4	1.8	− 1.3	1.7	− 1.4	2.0	− 1.4	1.6
Knee extension strength	− 2.1	1.0	− 2.6	1.5	− 2.6	1.3	− 2.5	1.4	− 2.7	1.4	− 2.9	1.5	− 2.9	1.4
Balance
Sensory organization test	− 0.5	2.1	0.0	2.0	− 0.1	2.0	− 0.6	2.2	− 1.0	2.0	− 1.0	2.2	− 1.2	2.7
Adaptive physical function
BOT-SF standardized score	− 1.0	0.9	− 0.9	1.0	− 0.8	0.9	− 1.0	0.9	− 0.9	0.9	− 1.0	1.1	− 0.9	1.1

Some participants were removed from the model due to missing some or all tests at each time point: Baseline, (n = 10) < 4 years old; 6-months, (n = 8) < 4 years old, (n = 13) no scheduled visit; 1 year (n = 6) < 4 years old, (n = 11) no scheduled visit, (n = 1) no show; 2 years, (n = 1) < 4 years old, (n = 4) no scheduled visit; 3 years, (n = 2) no scheduled visit, (n = 1) no show; 4 years, (n = 9) no scheduled visit, (n = 1) off study, (n = 1) COVID-19 cancellation; 5 years, (n = 6) no scheduled visit, (n = 5) COVID-19 cancellation, (n = 1) no show, (n = 1) off study

BOT-SF Bruininks-Oseretsky test of motor proficiency short form, n Number, ROM Range of motion, SD Standard deviation

^aFour participants were taking blood pressure medications at the time of assessment and were well controlled

Table 3.

Percentage of participants with craniopharyngioma whose performance on physical fitness measures is 1.5 SD below (*or above) age- and sex-specific normative values during a 5-year follow-up period

	Baseline (n = 84)		6-months (n = 73)		1 year (n = 76)		2 years (n = 89)		3 years (n = 91)		4 years (n = 83)		5 years (n = 81)
	%	95% CI	%	95% CI	%	95% CI	%	95% CI	%	95% CI	%	95% CI	%	95% CI
Anthropometrics
Waist to height ratio*	33.7	(28.5, 38.9)	43.1	(37.4, 48.8)	44.6	(38.8, 50.4)	40.4	(34.8, 46.0)	40	(34.4, 45.6)	49.4	(43.5, 55.3)	46.3	(40.5, 52.1)
Body mass index*	45.2	(39.4, 51.0)	63	(57.5, 68.5)	61.8	(56.3, 67.3)	56.2	(50.4, 62.0)	56	(50.2, 61.8)	65.1	(59.8, 70.4)	66.7	(61.5, 71.9)
Cardiopulmonary fitness
Resting systolic blood pressure*a	50	(44.1, 55.9)	49.3	(43.4, 55.2)	56.6	(50.8, 62.4)	41.6	(35.9, 47.3)	42.9	(37.2, 48.6)	39	(33.4, 44.6)	34.6	(29.3, 39.9)
Resting heart rate*	26.2	(21.7, 30.7)	24.7	(20.3, 29.1)	31.6	(26.5, 36.7)	31.5	(26.4, 36.6)	31.9	(26.8, 37.0)	39	(33.4, 44.6)	43.2	(37.5, 48.9)
Peak oxygen uptake	21.4	(17.5, 25.3)	26	(21.5, 30.5)	25	(20.6, 29.4)	27	(22.4, 31.6)	13.2	(10.5, 15.9)	14.5	(11.6, 17.4)	13.6	(10.8, 16.4)
Metabolism
Resting metabolic rate	8.3	(6.5, 10.1)	9.6	(7.6, 11.6)	10.5	(8.3, 12.7)	2.2	(1.7, 2.7)	7.7	(6.0, 9.4)	9.6	(7.6, 11.6)	7.4	(5.8, 9.0)
Flexibility
Sit and reach	17.9	(14.5, 21.3)	13.7	(10.9, 16.5)	11.8	(9.4, 14.2)	12.4	(9.9, 14.9)	16.5	(13.3, 19.7)	25.3	(20.9, 29.7)	22.2	(18.2, 26.2)
Dorsiflexion ROM	13.1	(10.4, 15.8)	9.6	(7.6, 11.6)	11.8	(9.4, 14.2)	23.6	(19.4, 27.8)	15.4	(12.3, 18.5)	26.5	(21.9, 31.1)	19.8	(16.1, 23.5)
Muscular strength
Hand grip strength	53.6	(47.8, 59.4)	54.8	(49.0, 60.6)	44.7	(38.9, 50.5)	44.9	(39.1, 50.7)	45.1	(39.3, 50.9)	51.8	(46.0, 57.6)	42.5	(36.8, 48.2)
Knee extension strength	54.2	(48.4, 60.0)	65.3	(60.0, 70.6)	69.3	(64.3, 74.3)	70.5	(65.6, 75.4)	72.5	(67.8, 77.2)	82.9	(79.6, 86.2)	81.3	(77.7, 84.9)
Balance
Sensory organization test	20.2	(16.4, 24.0)	19.2	(15.6, 22.8)	25	(20.6, 29.4)	30.3	(25.4, 35.2)	34.1	(28.8, 39.4)	38.6	(33.0, 44.2)	34.6	(29.3, 39.9)
Adaptive physical function
BOT-SF standardized score	29.8	(24.9, 34.7)	27.4	(22.7, 32.1)	28.9	(24.1, 33.7)	33.7	(28.5, 38.9)	30.8	(25.8, 35.8)	32.5	(27.4, 37.6)	25.9	(21.4, 30.4)

Some participants were removed from the model due to missing some or all tests at each time point: Baseline, (n = 10) < 4 years old; 6-months, (n = 8) < 4 years old, (n = 13) no scheduled visit; 1 year (n = 6) < 4 years old, (n = 11) no scheduled visit, (n = 1) no show; 2 years, (n = 1) < 4 years old, (n = 4) no scheduled visit; 3 years, (n = 2) no scheduled visit, (n = 1) no show; 4 years, (n = 9) no scheduled visit, (n = 1) off study, (n = 1) COVID-19 cancellation; 5 years, (n = 6) no scheduled visit, (n = 5) COVID-19 cancellation, (n = 1) no show, (n = 1) off study

BOT-SF Bruininks-Oseretsky test of motor proficiency short form, CI Confidence interval, n Number, % cent, ROM Range of motion, SD Standard deviation

^aFour participants were taking blood pressure medications at the time of assessment and were well controlled

*Above 1.5 SD

Table 4.

Percentage of participants with craniopharyngioma whose performance on physical fitness measures is 2.0 SD below (*or above) age- and sex-specific normative values during a 5-year follow-up period

	Baseline (n = 84)		6-months (n = 73)		1 year (n = 76)		2 years (n = 89)		3 years (n = 91)		4 years (n = 83)		5 years (n = 81)
	%	95% CI	%	95% CI	%	95% CI	%	95% CI	%	95% CI	%	95% CI	%	95% CI
Anthropometrics
Waist to height ratio*	22.9	(18.8, 27.0)	34.7	(29.4, 40.0)	29.7	(24.8, 34.6)	27	(22.4, 31.6)	31.1	(26.1, 36.1)	33.7	(28.5, 38.9)	38.8	(33.2, 44.4)
Body mass index*	29.8	(24.9, 34.7)	37	(31.5, 42.5)	36.8	(31.4, 42.2)	30.3	(25.4, 35.2)	39.6	(34.0, 45.2)	39.8	(34.2, 45.4)	38.3	(32.8, 43.8)
Cardiopulmonary fitness
Resting systolic blood pressure*a	48.8	(42.9, 54.7)	46.6	(40.8, 52.4)	52.6	(46.8, 58.4)	38.2	(32.7, 43.7)	38.5	(33.0, 44.0)	32.9	(27.7, 38.1)	30.9	(25.9, 35.9)
Resting heart rate*	19	(15.4, 22.6)	20.5	(16.7, 24.3)	26.3	(21.8, 30.8)	24.7	(20.3, 29.1)	20.9	(17.0, 24.8)	32.9	(27.7, 38.1)	29.6	(24.7, 34.5)
Peak oxygen uptake	11.9	(9.4, 14.4)	17.8	(14.4, 21.2)	13.2	(10.5, 15.9)	18	(14.5, 21.5)	6.6	(5.2, 8.0)	9.6	(7.6, 11.6)	4.9	(3.8, 6.0)
Metabolism
Resting metabolic rate	3.6	(2.8, 4.4)	2.7	(2.1, 3.3)	5.3	(4.1, 6.5)			2.2	(1.7, 2.7)	6	(4.7, 7.3)	1.2	(0.9, 1.5)
Flexibility
Sit and reach	11.9	(9.4, 14.4)	6.8	(5.3, 8.3)	3.9	(3.0, 4.8)	5.6	(4.4, 6.8)	8.8	(6.9, 10.7)	12	(9.5, 14.5)	9.9	(7.8, 12.0)
Dorsiflexion ROM	7.1	(5.6, 8.6)	5.5	(4.3, 6.7)	6.6	(5.2, 8.0)	15.7	(12.6, 18.8)	8.8	(6.9, 10.7)	16.9	(13.6, 20.2)	12.3	(9.8, 14.8)
Muscular strength
Hand grip strength	40.5	(34.9, 46.1)	43.8	(38.0, 49.6)	35.5	(30.1, 40.9)	33.7	(28.5, 38.9)	28.6	(23.8, 33.4)	37.3	(31.8, 42.8)	37.5	(32.0, 43.0)
Knee extension strength	39.8	(34.2, 45.4)	52.8	(47.0, 58.6)	58.7	(53.0, 64.4)	52.3	(46.5, 58.1)	61.5	(56.0, 67.0)	73.2	(68.6, 77.8)	73.8	(69.3, 78.3)
Balance
Sensory organization test	14.3	(11.4, 17.2)	11	(8.7, 13.3)	19.7	(16.0, 23.4)	23.6	(19.4, 27.8)	26.4	(21.8, 31.0)	31.3	(26.3, 36.3)	25.9	(21.4, 30.4)
Adaptive physical function
BOT-SF standardized score	14.3	(11.4, 17.2)	12.3	(9.8, 14.8)	15.8	(12.7, 18.9)	13.5	(10.8, 16.2)	14.3	(11.4, 17.2)	18.1	(14.6, 21.6)	12.3	(9.8, 14.8)

Some participants were removed from the model due to missing some or all tests at each time point: Baseline, (n = 10) < 4 years old; 6-months, (n = 8) < 4 years old, (n = 13) no scheduled visit; 1 year (n = 6) < 4 years old, (n = 11) no scheduled visit, (n = 1) no show; 2 years, (n = 1) < 4 years old, (n = 4) no scheduled visit; 3 years, (n = 2) no scheduled visit, (n = 1) no show; 4 years, (n = 9) no scheduled visit, (n = 1) off study, (n = 1) COVID-19 cancellation; 5 years, (n = 6) no scheduled visit, (n = 5) COVID-19 cancellation, (n = 1) no show, (n = 1) off study

BOT-SF Bruininks-Oseretsky test of motor proficiency short form, CI Confidence interval, n Number, % cent, ROM Range of motion, SD Standard deviation

^aFour participants were taking blood pressure medications at the time of assessment and were well controlled

*Above 2.0 SD

Figure 1 shows change in impairment status from the 2 to 5 years’ time point. Both BMI and knee extension strength had the largest proportion of participants who were impaired at both 2 and 5 years (51.9% and 62.8%, respectively). Resting HR had the highest proportion of participants who were not impaired at 2 years but became impaired at 5 years (26.3%). Systolic BP (23.8%) and performance on the BOT-SF (18.5%) had the highest proportions whose status changed from impaired at 2 years to not impaired at 5 years. Excluding the participants who started their treatment over 1 year after diagnosis did not substantially change the mean z-score, percent impaired, or percent who changed their impairment status (Online Resource Tables 2–4, and Online Resource Fig. 1). Additionally, excluding the participants who did not complete every time-point did not substantially change the mean z-score, percent impaired, or percent who changed their impairment status (Online Resource Tables 5–7, and Online Resource Fig. 2).

Fig. 1.

Percentage of participants with craniopharyngioma whose impairment status (below or above 1.5 standard deviations from normative values) changed, or whose impairment status stayed the same, from 2-year follow-up to 5-year follow-up. WHR Waist to height ratio, BMI body mass index, BP Blood pressure, HR Heart rate, BOT-SF Bruinink-Oseretsky test of motor proficiency test, SOT Sensory Organization Test. A total of 16 patients were excluded from the analysis due to missing the 2-year follow-up, the 5-year follow-up, or both

Discussion

The results of this study indicate that a majority of children with craniopharyngioma have BMI and fitness abnormalities at diagnosis that do not normalize by 5 years after PRT. The prevalence of impairment is highest for BMI and muscular strength and increases over time for both. Although the proportion with impaired cardiopulmonary fitness decreases over time, it is particularly concerning that more than one third of our cohort had elevated resting BP and HR 5 years after treatment. These data indicate that children with craniopharyngioma may benefit from interventions designed to improve BMI, strength, and resting indicators of cardiopulmonary fitness.

To our knowledge, this is the first study to report the results of a comprehensive physical fitness assessment in children with craniopharyngioma from treatment (baseline) through 5 years of follow-up. Although our study is consistent with others demonstrating children with craniopharyngioma have BMI impairments [29] and impaired cardiopulmonary fitness [3], our data describing that over 80% of this population have either impaired upper or lower body muscular strength 5 years after treatment is novel. Low grip strength among children in the general population is associated with future cardiovascular disease and metabolic risk [30]. In a longitudinal cohort of Swedish men (N = 1,078,685), those who had higher knee extension strength as adolescents had lower rates of cardiovascular disability as adults [31]. In addition, muscular strength deficits in children continue into adulthood [32], where they remain associated with cardiometabolic disease and physical disability [33].

Nearly two-thirds of 5-year survivors of craniopharyngioma in this study had elevated BMI values. Previous data show that among 65 children who had hypothalamus sparing surgery, BMI values at 6 months (z-score = 1.56) and 12 months (z-score = 1.89) post-surgery were similar to our participants, especially those with craniotomies [29], well into the obese range. Another group reported that among 45 children treated for craniopharyngioma, mean BMI z-score 7 years after diagnosis was 2.10 ± 1.70 [13]. This is slightly higher than the mean among our participants with 2 years less of follow-up, but supports our finding of a weight gain trend during survivorship. Obese adolescents often become more obese with age [34], and have higher rates of cardiometabolic morbidity [35] and mortality [36] in adulthood. Data from otherwise healthy adolescents (n = 1782) show that those with obesity were 4.51 (95% CI 2.83–7.19) times more likely to have left ventricular hypertrophy, 2.24 (95% CI 1.46–3.45) times more likely to have elevated systolic BP, and 2.10 (95% CI 1.06–4.17) times more likely to have elevated diastolic BP than those without obesity [37]. Our cohort is already at risk for cardiovascular disability from muscular strength impairment; obesity could compound this risk. Approximately one third of the participants did not have impaired BMI at 24 or 60 months and less than 4 percent went from impaired to not impaired. Our participants who had craniotomies had higher BMI values than those who did not, so less invasive surgery may be one of many factors, including normal tissue-sparing PRT, in preventing obesity among some survivors 2 and 5 years after PRT.

In fact, these children already have early markers of future cardiovascular disease risk. A third had elevated systolic BP, and nearly half had elevated resting HR at 5 years of follow-up. Data from multiple studies (males, N sizes ranging from 2104 to 803,505) indicate that a one SD higher systolic BP in adolescence increases risk for ischemic stroke (Hazard Ratio [HR], 1.04; 95% CI 1.02–1.07) and hemorrhagic stroke (HR, 1.12; 95% CI 1.07–1.17) in adulthood [38], that both elevated systolic and diastolic BPs in adolescence increase risk for cardiovascular mortality in adulthood [39], and that elevated resting HR in adolescence is associated with heart failure, dilated cardiomyopathy [40], and cardiovascular and all-cause mortality [41]. Fortunately, interventions to address strength deficits improve markers of vascular flow in lean children [42], measures of glycemic control in children with insulin resistance [43], function in children with cerebral palsy, [44] frequency domain components of HR variability in children with cystic fibrosis [45], and both skeletal muscle mass and visceral adiposity in children and adolescents with obesity [46]. Interventions to promote weight loss such as diet, medications, and resistance and aerobic training, are also beneficial in pediatric populations [47].

Amongst our cohort, whose radiation exposure was proton based, both RMR and peak oxygen uptake improved during this 5-year study. These results differ from those presented for a small cohort (N = 8) of children with craniopharyngioma treated with traditional radiotherapy [48]. The RMR was significantly impaired for obese participants with craniopharyngioma compared to predicted values (mean kcals ± SD; 1541 ± 112.6 vs. 1809 ± 151.8, p = 0.01) [48]. This was a 17% decrease compared to obese controls who had no significant difference compared to predicted values (1647 ± 33.2 vs. 1652 ± 40.2, p = 0.8) [48]. Our cohort’s improved RMR could indicate that the normal tissue-sparing PRT may reduce hypothalamic dysfunction, which is postulated to be associated with low RMR in this population [49]. Additionally, our peak oxygen uptake data agree with a study that evaluated craniopharyngioma survivors treated with surgery alone where relative peak oxygen uptake 5.7 years post-surgery was 20–25% lower than among healthy controls [3]. Participants in our study, all of whom had PRT, had a mean z-score at 5 years of − 0.5 ± 0.9, 19.1% lower than expected compared to normative values. Although this comparative study has a small sample size who received traditional radiotherapy, it appears that in addition to sparing tissue responsible for controlling metabolism, PRT exposure does not adversely affect peak oxygen uptake beyond that conferred by surgical resection.

This study is not without limitations. First, due to the rarity of craniopharyngioma, our study’s sample size was relatively small (n = 94), so we were unable to perform multivariable analyses on how treatment impacted the physical function status of our participants. It is important for future studies to examine which clinical and treatment parameters impact the function of children with craniopharyngioma. Second, our study did not collect lifestyle covariates such as physical activity or diet information. As it stands, it is unclear what impacts physical function the most, treatment or lifestyle. Future studies should consider how the interaction between lifestyle and treatment impact physical function of children with craniopharyngioma. Third, there was some missing outcome data (ranging from 4 to 22% depending on timepoint), which may have biased our results. Missing data were most often due to a child not being able to perform a task, thus estimates of impairment prevalence are likely low. All tests were attempted with every participant, even the very young, but there were times when a test was not appropriate due to age. Alternatively, because most of the measured outcomes were effort based, there may be some misclassification of impairment status. Also, it can be difficult to interpret BMI and physical performance changes during puberty. This could lead to misclassification of the outcomes.

Our findings are the first to document fitness from treatment through 5 years of survival in children with craniopharyngioma. The high proportion and rising trajectory of poor strength, obesity and elevated resting HR and BP over 5-years of follow-up, particularly for patients who had craniotomies, likely increases risk for future cardiovascular disease in these children. Multifaceted interventions, including a combination of pharmaceutical (weight loss medications), behavioral (diet, exercise), and caregiver education are needed to address these impairments.

Supplementary Information

Below is the link to the electronic supplementary material.

Author contributions

All authors contributed to the study conception and design. Acquisition of data, interpretation of data, drafting the article, and final approval was performed by Robyn Partin and Matthew Wogksch. Analysis of data, revising the article, and final approval was performed by Robyn Partin. Interpretation of data, revising the article, and final approval was performed by Jason Halford and Heather Conklin. Conception and design, revising the article, and final approval was performed by Daniel Indelicato. All authors read and approved the final manuscript.

Funding

This work was supported by the American Lebanese Syrian Associated Charities (ALSAC) and the St. Jude Cancer Center Support [CORE] Grant [P30 CA 21765 (Roberts)].

Data availability

Data are available upon request from the corresponding author.

Declarations

Competing interest

The authors have no relevant financial or non-financial interests to disclose.

Ethics approval

The study protocol was approved by the St. Jude Children Research Hospital Institutional Review Board.

Consent to participate

Written informed consent was obtained from all participants.