Figure 4.
Impaired autophagy in structured cells contribute to autophagy-mediated airway inflammation in obesity asthma. (A) Experimental timeline. WT or atg5−/− bone-marrow (BM) cells were adaptively transferred into irradiated WT or atg5−/− mice. The mice were fed with HFD, and then immunized with HDM. A part of the BM cells was isolated from CAG-EGFP transgenic mice to assess the reconstitution rate. (B) Reconstitution rate. (C) Lung resistance (RL) and dynamic compliance (Cdyn) (D) Differential cell counts in BALF (n = 8–9/group). TCC, total cell count; MAC, macrophages; Ly, lymphocytes; Eo, eosinophils; Neut, neutrophils. (E) Hematoxylin and eosin-staining. Original magnification: 100 × . (F) PAS staining. (G) Quantifications of lung histopathology (n = 5/group). Data are representative of two independent experiments. Data are expressed as the mean ± SEM. P-values were calculated using the Student’s t test (atg5−/− (host) + atg5−/− BM vs. WT (host) + atg5−/−BM, and atg5−/− (host) + WT BM vs. WT (host) + WT BM). *, P < 0.05, **, P < 0.01, ***, P < 0.001.