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. 2022 Aug 1;10(8):1853. doi: 10.3390/biomedicines10081853

Figure 4.

Figure 4

Effects of acute CBD exposure on mBDNF and TrkB receptor protein levels in the mPFC. Rats were treated with a single injection of CBD 5 mg/kg, 15 mg/kg, or 30 mg/kg and killed one hour after treatment. Protein levels of BDNF (a), phospho(p)-TrkBY706 (b), TrkB (c), and of the ratio pTrkB/TrkB (d) measured in the homogenate of mPFC are expressed as percentages of saline-treated rats. Below the graphs representative immunoblots are shown for mBDNF (14 kDa), pTrkB Y706 (145 kDa), TrkB (145 kDa), and β-Actin (43 kDa) proteins. Bar graphs represent the mean ± SEM from eight independent determinations for each experimental group. Ordinary one-way ANOVA followed by Tukey’s multiple comparisons test, or Kruskal-Wallis one-way ANOVA for ranks followed by Dunn’s multiple comparisons test for non-normally distributed data. * p < 0.05 vs. saline-treated rats. Saline n = 8, CBD 5 mg/kg n = 8 CBD 15 mg/kg n = 8, CBD 30 mg/kg n = 8 (mBDNF n = 7).