Figure A2.

MaR1 improved the osteogenic function of osteoblasts in high-glucose medium by activating NRF2 signaling. (A) The osteoblasts that were engaged in DNA replication were examined using EdU staining. Red, EdU-positive nuclei; blue, all nuclei. Scale bar = 100 μm. (B) The quantitative evaluation of cells that were EdU-positive. (C) Western blot analysis was used to determine the protein levels of COLI, ALP, and RUNX2 after seven days of osteogenic induction. (D) The quantification analysis of the Western blotting gray-value expression. (E) An assessment of ALP activity seven days following osteogenic induction. Scale bar = 200 μm. Data are presented as means ± SDs from three separate experiments (n = 3). ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. SiNC: siRNA negative control; siNRF2: siRNA of NRF2.