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. 2022 Aug 11;11(16):2490. doi: 10.3390/cells11162490

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Conditioned media from HCT116 and LS174T cells modulated different aspects of the fibroblasts phenotype, mostly independently of KRAS. Secreted factors from HCT116 cells were able to increase fibroblast α-SMA expression and TGFβ1 secretion as well as to downregulate MMPs expression. Secreted factors from LS174T cells led to an increase in fibroblast migration and a decrease in collagen contraction capacities as well as the downregulation of MMP14 expression. Independent of KRAS, secreted factors from both cell lines increased the levels of fibroblast-secreted HGF. A KRAS dependency was only found regarding the capacity to regulate migration, in the case of HCT116 cells, and α-SMA and MMP2 expression in the case of LS174T cells.