Figure 13.

Upon treatment with UA, nephrin co-localization with early endosomal marker EEA1 was increased. Podocytes cultured for 7 d in normal (5.5 mM, NG) or high (25 mM, HG) glucose were incubated for 24 h with 10 µM UA. Representative confocal microscopy images of double staining against nephrin and EEA-1 (A). Quantification of nephrin and EEA-1 co-localization (B) Experiments were performed in duplicate and 500 cells were analyzed per experiment (n = 3). ANOVA test was used to calculate p values. *** p < 0.001.