Volume 181, no. 12, p. 3705–3709, 1999. Page 3708: Table 2 should appear as shown below.
TABLE 2.
Bond cleavage frequencies and specific activities of the E. chrysanthemi 3937 endopectate lyases PelA, PelI, PelL, PelD, and PelB acting on oligogalacturonates of defined lengthsa
| Enzyme | nb | Oligogalacturonatec | Activity (U · mg−1) |
|---|---|---|---|
| PelA | 3 | G — G — G | 0.6 |
| 100 | |||
| 4 | G — G — G — G | 17 | |
| 100 | |||
| 5 | G — G — G — G — G | 12 | |
| 23 77 | |||
| 6 | G — G — G — G — G — G | 15 | |
| 33 6 61 | |||
| 7 | G — G — G — G — G — G — G | 23 | |
| 70 9 2 19 | |||
| 8 | G — G — G — G — G — G — G — G | 25 | |
| 62 19 6 2 11 | |||
| PelI | 3 | G — G — G | 0.6 |
| 100 | |||
| 4 | G — G — G — G | 11 | |
| 62 38 | |||
| 5 | G — G — G — G — G | 87 | |
| 43 22 34 | |||
| 6 | G — G — G — G — G — G | 252 | |
| 74 23 3 | |||
| 7 | G — G — G — G — G — G — G | 504 | |
| 4 91 5 | |||
| 8 | G — G — G — G — G — G — G — G | 630 | |
| 2 33 61 4 | |||
| PelL | 3 | G — G — G | 0 |
| 4 | G — G — G — G | 0.02 | |
| 100 | |||
| 5 | G — G — G — G — G | 0.22 | |
| 28 72 | |||
| 6 | G — G — G — G — G — G | 2.2 | |
| 64 34 2 | |||
| 7 | G — G — G — G — G — G — G | 3.5 | |
| 4 91 5 | |||
| 8 | G — G — G — G — G — G — G — G | 3 | |
| 3 38 57 2 | |||
| PelD | 3 | G — G — G | 13 |
| 100 | |||
| 4 | G — G — G — G | 418 | |
| 100 | |||
| 5 | G — G — G — G — G | 310 | |
| 8 92 | |||
| 6 | G — G — G — G — G — G | 268 | |
| 3 3 94 | |||
| 7 | G — G — G — G — G — G — G | 250 | |
| 4 7 16 73 | |||
| 8 | G — G — G — G — G — G — G — G | 222 | |
| 1 14 10 75 | |||
| PelB | 3 | G — G — G | 40 |
| 100 | |||
| 4 | G — G — G — G | 2,400 | |
| 43 57 | |||
| 5 | G — G — G — G — G | 3,660 | |
| 69 31 | |||
| 6 | G — G — G — G — G — G | 4,070 | |
| 10 52 38 | |||
| 7 | G — G — G — G — G — G — G | 3,780 | |
| 2 6 62 30 | |||
| 8 | G — G — G — G — G — G — G — G | 3,160 | |
| 11 67 22 |
a
Conditions of this experiment included the following: 0.5 mM oligogalacturonate (n = 3 to 8), 1 mM CaCl2, and 20 mM Tris-HCl (pH 8.0) (for PelA, PelD, and PelL) or 20 mM amino-methyl-propanol–HCl (pH 9.0) (for PelB and PelI) at 37°C. Product analysis was done as described in Materials and Methods.
b
n, degree of polymerization.
c
Bond cleavage frequencies are given in percentages. The reducing ends of the oligogalacturonates are printed in bold type.