Effects of L7Gn on the behavior of sleep-deprived mice in TST (B) and FST (C). (A) Experimental design. L7Gn was dissolved in 70% PEG-saline, and FXT was dissolved in 0.9% physiological saline. Mice were administered vehicle (70% PEG), L7Gn (0.3, 1, and 3 mg/kg), or FXT (20 mg/kg) once daily for four–five days, followed by behavior tests. (B) Body weight: (two-way ANOVA: (Interaction: F (20, 150) = 1.258, p = 0.2165; Days: F (4, 150) = 15.18, p < 0.0001; Group: F (5, 150) = 16.64, p < 0.0001)) (C) TST: (one-way ANOVA: F (5, 38) = 7.627, p < 0.001) (D) FST: (one-way ANOVA: F (5, 35) = 3.76, p = 0.0079). Data are presented as mean ± SEM (n = 5–8/group). * p < 0.05, significantly different from the control group. # p < 0.05, significantly different from the vehicle group. CTL, control; Veh, vehicle; FXT, fluoxetine.