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. 2022 Aug 12;14(16):3314. doi: 10.3390/nu14163314

Figure 6.

Figure 6

Effect of L7Gn on the BDNF expression and the phosphorylation of TrkB, ERK, and CREB in the hippocampus of sleep-deprived mice. L7Gn was dissolved in 70% PEG-saline. Mice were administered vehicle (70% PEG) or L7Gn (0.3, 1, and 3 mg/kg) once daily for five days. Western blotting and quantification of expression levels of (A) BDNF, (B) p-TrkB, (C) p-ERK, and (D) p-CREB. The expression level of BDNF was normalized against GAPDH and those of p-TrkB, p-ERK, and p-CREB were normalized against their unphosphorylated form. (A) BDNF: (one-way ANOVA: F (4, 15) = 24.33, p < 0.0001) (B) p-TrkB: (one-way ANOVA: F (4, 18) = 7.58, p = 0.0009) (C) p-ERK: (one-way ANOVA: F (4, 20) = 10.01, p = 0.0001) (D) p-CREB: (one-way ANOVA: F (4, 20) = 6.254, p = 0.0020). Data are presented as mean ± SEM (n = 4–5/group). * p < 0.05, significantly different from the control group. # p < 0.05, significantly different from the vehicle group. CTL, control; Veh, vehicle; FXT, fluoxetine.