Table 1.

Physicochemical properties of the LNPs examined in screening A.

Entry	%CL	%DOPE	%PEG	NaCl Conc. (mM)	CL	PEG	ζ-Average (nm)	PdI	ζ-Potential (mV)	%RNA Encapsulation
A-1	40	40	0.5	0	CL7H6	PEG-DMG	112	0.20	−3.2	90.3
A-2	50	10	0.5	0	CL4H6	PEG-DMG	121	0.19	−2.3	99.2
A-3	60	10	1	0	CL7H6	PEG-DSG	104	0.15	−2.3	91.0
A-4	60	40	1.5	0	CL7H6	PEG-DMG	128	0.41	−0.6	89.8
A-5	40	25	1.5	0	CL4H6	PEG-DSG	88	0.30	−1.5	99.2
A-6	60	40	0.5	200	CL4H6	PEG-DSG	155	0.13	1.9	98.8
A-7	50	25	1	200	CL4H6	PEG-DMG	138	0.12	−1.1	99.0
A-8	40	10	1.5	200	CL7H6	PEG-DMG	128	0.20	−2.6	85.0
A-9	50	25	1	200	CL7H6	PEG-DSG	171	0.15	−2.7	88.0
A-10	40	40	1	400	CL4H6	PEG-DMG	216	0.08	2.5	99.0
A-11	60	10	1.5	400	CL4H6	PEG-DSG	547	0.19	−1.4	89.2
A-12	50	40	1.5	400	CL7H6	PEG-DSG	108	0.07	−1.7	88.3
A-13	60	25	0.5	400	CL7H6	PEG-DMG	501	0.12	−1.2	91.2
A-14	40	10	0.5	400	CL4H6	PEG-DSG	754	0.17	−10.3	72.4