The initial and final samples included in this study. Pregnant women with gestational diabetes mellitus (GDM group), with GDM diagnosed in the first trimester, (Early-GDM group) and women with normal glucose tolerance in pregnancy (Non-GDM group), according the two-step diagnosis criteria (18), are indicated. The Post-COVID-19 Missed- and New-Early-GDM groups were identified according to the recommendations of Spain (4) for the Early-GDM diagnosis during pandemic, first trimester fasting plasma glucose (1t-FPG) < or ≥100 mg/dL, respectively. The post-COVID-Missed-GDM and post-COVID-New-GDM groups were identified according to the recommendations of Australia (1), Italy (2), Spain (3), and UK (5), FPG at 24–28 weeks of pregnancy (2t-FPG) <84, <92, <95 and <100 mg/dL, respectively or ≥92, ≥95 and ≥100 mg/dL, respectively. GDM, gestational diabetic mellitus; NDDG, National Diabetes Data Group; post-COVID, post-coronavirus disease.