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. 2022 Aug 10;14(8):1665. doi: 10.3390/pharmaceutics14081665

Figure 1.

Figure 1

KCl stimulation increases the expression of HSP90a and the co-chaperone endoplasmin in murine brain endothelial cells. bEnd.3 mouse endothelial cells cultured in the presence of astrocyte-conditioned media were treated with 60 mM KCl or aCSF for 5 min. The KCl pulse (60 mM) mimicked the conditions during cortical spreading depression. aCSF was used as vehicle control. The cells were then lysed in lysis buffer and subjected to proteomic analysis. The graphs show proteomic spectral counts for HSP70 isoforms (A), HSP90 isoforms (B), and HSP90 co-chaperones (C) detected by unlabeled proteomic approaches. (A) No significant differences were observed in the expression of HSP70 isoforms between KCl vs. aCSF-treated cells. (B) The KCl pulse significantly increased the expression of HSP90a, but not HSP90b compared to aCSF control. (C) Significant increase in the expression of endoplasmin was detected in KCl-treated cells, compared to aCSF ones, but there was no significant difference in the expression of CDC37 between KCl and aCSF-treated cells. ns = non-significant, * p < 0.05, ** p < 0.01, as assessed by two-way ANOVA, Sidak–Holms corrected t-test. Values are mean ± SEM (n = 4). Circles and squares indicate individual subjects.