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. Author manuscript; available in PMC: 2022 Aug 29.
Published in final edited form as: Oncogene. 2011 Jun 13;31(1):116–127. doi: 10.1038/onc.2011.217

Figure 2.

Figure 2

YY1 positively regulates BRCA1 expression. (a) YY1 positively regulated the endogenous BRCA1 expression as revealed by RT–PCR analysis in YY1-inducible U2OS cells (by tetracycline withdrawal, –Tet; Sui et al., 2004) and YY1 overexpression in HEK293 and HeLa cells. (b) Ectopic expression of YY1 in HeLa cells increased BRCA1 as revealed by western blot analysis. (c) Increased YY1 in U2OS cells induced BRCA1 protein level in the total protein lysates and nuclear extracts. (d) Reduced levels of YY1 and BRCA1 proteins in MDA-MB-231 and T47D breast cancer cell lines after YY1 knockdown by two lentiviral-based shRNA constructs that are specific to YY1 (Sh1 and Sh2) compared with shRNA control vector pLKO.1. (e) YY1 overexpression caused the increased G0-G1 population in the cell cycle in MDA-MB-231 and Tet-regulated U2OS cells.