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. 2022 Jul 12;13(4):e01639-22. doi: 10.1128/mbio.01639-22

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Copyright © 2022 Delgado et al.

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FIG 2 — Pyrococcus furiosus thioredoxin (PfTrx) as a scaffold for presentation of extracellular loops (ECLs). (A) ModWeb prediction of the β-barrel of Tp BamA (TP0326) (12) with ECL4 shown in red. (B) Phyre2 prediction of the PfTrx structure showing the insertion site (green) for ECLs (here BamA ECL4) and a linear map of the PfTrx^BamA/ECL4 construct with N-His- and C-Avi- tags shown in pink and yellow, respectively. (C) Reactivity of PfTrx^BamA/ECL4 and PfTrx with IRS, HSS, NRS, NHS, mouse anti-Avi-Tag, and rat anti-BamA ECL4 antiserum. The highly antigenic lipoprotein, Tpp17, was used as a positive control. (D) Supernatant (Sup) and elution (Elu) fractions from Protein G pull-downs of PfTrx^BamA/ECL4 and PfTrx with IRS or NRS immunoblotted with Avi-Tag Abs.