FIG 3.

TREM2 expression alters cytokine production in M. tuberculosis-infected macrophages. (A to D) THP-NT, THP-ΔTREM2, THP-Vector, and THP-TREM2+ macrophages were infected with M. tuberculosis (MOI = 10), and culture supernatants were collected at 24 h postinfection. Levels of (A) TNF-α, (B) IL-1β, (C) IL-10, and (D) IFN-β were measured using human ELISA kits. (E and F) THP-Vector and THP-TREM2+ macrophages were mock treated or pretreated with R406 (SYK inhibitor; 10 μM), anti-IFNAR1 (2.5 μg/mL), SN-011 (STING inhibitor; 1 μM), and RU.521 (cGAS inhibitor; 10 μg/mL) for 2 h and 6 h (in the case of SN-011), prior to M. tuberculosis infection (MOI = 10). Culture supernatants were collected at 24 h postinfection and levels of (E) TNF-α and (F) IL-1β were measured. Error bars represent the mean ± SD of three independent biological replicates.