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. 2022 Aug 4;13(4):e01456-22. doi: 10.1128/mbio.01456-22

FIG 5.

FIG 5

TREM2 facilitates intracellular survival of M. tuberculosis. (A) THP-NT, THP-ΔTREM2, THP-Vector, and THP-TREM2+ macrophages were infected with the autoluminescent Mtb-lux strain at an MOI of 10. Luminescence signal (RLU) representing viable M. tuberculosis was analyzed at the indicated time points. (B) THP-1 macrophages and (C) hMDMs were mock treated or pretreated with anti-TREM2 antibody for 20 min, followed by infection with Mtb-lux (MOI = 10), and RLU were analyzed at the indicated time points postinfection. (D) THP-Vector and THP-TREM2+ macrophages were mock treated or pretreated with R406 (SYK inhibitor; 10 μM), anti-IFNAR1 (2.5 μg/mL), SN-011 (STING inhibitor; 1 μM), or RU.521 (cGAS inhibitor; 10 μg/mL) for 2 h and 6 h (in the case of SN-011) and infected with Mtb-lux (MOI = 10). RLU were analyzed at day 4 postinfection. Error bars in this figure represent the mean ± SD from three independent biological replicates.