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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Hepatology. 2021 Jun 21;74(3):1560–1577. doi: 10.1002/hep.31831

Fig. 1. Disruption of CD47 in MSCs exacerbates IR-induced liver injury and augments proinflammatory mediators.

Fig. 1.

WT mice were adoptive transferred MSCs or CD47-deficient MSCs (1x106) 24h prior to liver ischemic insult. (A) The CD47 expression was detected by Western blot assay in ischemic livers. The representative of three experiments. (B) Representative histological staining (H&E) of ischemic liver tissue (n=4-6 mice/group) and Suzuki's histological score. Scale bars, 200μm and 50μm. (C) Liver function was evaluated by serum ALT levels (IU/L) (n=4-6 samples/group). (D) Immunohistochemistry staining of NLRP3 in ischemic livers (n=4-6 mice/group). Scale bars, 50μm and 20μm. (E) qRT-PCR analysis of IL-1β, TNF-α, IL-6, CXCL-2, and CXCL-10 in ischemic livers (n=3-4 samples/group). (F) ELISA analysis of serum IL-1β levels (n=3-4 samples/group). All data represent the mean±SD. *p<0.05, **p<0.01, ***p<0.001.