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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Hepatology. 2021 Jun 21;74(3):1560–1577. doi: 10.1002/hep.31831

Fig. 4. Disruption of myeloid Notch1 in MSC-treated livers enhances NEK7/NLRP3 function in IR-stressed livers.

Fig. 4.

The Notch1FL/FL and Notch1M-KO mice were adoptive transferred MSCs or CD47-deficient MSCs (1x106) 24h prior to liver ischemic insult. (A) Representative histological staining (H&E) of ischemic liver tissue (n=4-6 mice/group) and Suzuki's histological score. Scale bars, 200μm and 50μm. (B) Serum ALT levels (IU/L) (n=4-6 samples/group). (C) Immunofluorescence staining of CD11b+ macrophages in ischemic livers (n=4-6 mice/group). Quantification of CD11b+ macrophages, Scale bars, 50μm. (D) Immunohistochemistry staining of Ly6G+ neutrophils in ischemic livers (n=4-6 mice/group). Quantification of Ly6G+ neutrophils, Scale bars, 50μm. (E) Western blot analysis and relative density ratio of NICD, NEK7, NLRP3, ASC, and cleaved caspase-1 ischemic livers. (F) qRT-PCR analysis of IL-1β, TNF-α, IL-6, CXCL-2, and CXCL-10 (n=3-4 samples/group). All Western blots represent three experiments, and the data represent the mean±SD. *p<0.05, **p<0.01, ***p<0.001.